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Enhancing scleroglucan production by Sclerotium rolfsii WSH-G01 through a pH-shift strategy based on kinetic analysis.

08:00 EDT 3rd September 2019 | BioPortfolio

Summary of "Enhancing scleroglucan production by Sclerotium rolfsii WSH-G01 through a pH-shift strategy based on kinetic analysis."

As a stable microbial polysaccharide, scleroglucan has extensive application in the food, medicine, and cosmetics industries. However, its large-scale industrial application is limited by its high production cost, low yield, long production time, etc. This study aims to enhance scleroglucan production by Sclerotium rolfsii WSH-G01. Based on the analysis of batch fermentation kinetics parameters, a pH-shift strategy was adopted. Through systematic kinetics analysis, a 32.4 g/L scleroglucan was accomplished. The kinetic model of the pH-shift batch fermentation process was established using a logistic equation, Luedeking-Piret equation, and a Luedeking-Piret-like equation. As decreased glucose concentration could cause decreased scleroglucan synthesis rates during the batch fermentation process, 30 g/L glucose was fed in the later phase of fermentation. As a result, scleroglucan production increased to 42 g/L, with a productivity of 0.5 g/L·h. Thus, the pH-shift strategy and feeding approach could be useful for industrial scleroglucan production.

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Name: Bioresource technology
ISSN: 1873-2976
Pages: 122098

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