Label-free, real-time on-chip sensing of living cells via grating-coupled surface plasmon resonance.

08:00 EDT 3rd September 2019 | BioPortfolio

Summary of "Label-free, real-time on-chip sensing of living cells via grating-coupled surface plasmon resonance."

The application of nanotechnologies to address biomedical questions is a key strategy for innovation in biomedical research. Among others, a key point consists in the availability of nanotechnologies for monitoring cellular processes in a real-time and label-free approach. Here, we focused on a grating-coupled Surface Plasmon Resonance (GC-SPR) sensor exploiting phase interrogation. This sensor can be integrated in a microfluidic chamber that ensures cell viability and avoids cell stress. We report the calibration of the sensor response as a function of cell number and its application to monitor cell adhesion kinetics as well as cell response to an external stimulus. Our results show that GC-SPR sensors can offer a valuable alternative to prism-coupled or imaging SPR devices, amenable for microfluidic implementation.


Journal Details

This article was published in the following journal.

Name: Biophysical chemistry
ISSN: 1873-4200
Pages: 106262


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