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Although clade A phosphatase 2Cs (PP2CAs) are well known to regulate abscisic acid (ABA) signaling, few members of this family have been identified in alfalfa so far. Here, the isolation and characterization of the gene MsPP2CA1 from alfalfa is described. Its transcription was found to be highly inducible by treatment with abscisic acid, salt, hydrogen peroxide and polyethylene glycol. The constitutive expression of MsPP2CA1 in Arabidopsis thaliana seedlings mitigates root growth imposed by either salinity or oxidative stress, while also raising the level of sensitivity to ABA during germination and early seedling development, and promoting stomatal closure. In transgenic plants, many ABA-dependent stress-responsive genes were activated, and the expressions of catalase and peroxidase which involved in reactive oxygen scavenging were promoted. MsPP2CA1 is suggested as a candidate for the genetic manipulation of salinity tolerance in legume species.
This article was published in the following journal.
Name: Plant physiology and biochemistry : PPB
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The introduction of new genes into cells for the purpose of treating disease by restoring or adding gene expression. Techniques include insertion of retroviral vectors, transfection, homologous recombination, and injection of new genes into the nuclei of single cell embryos. The entire gene therapy process may consist of multiple steps. The new genes may be introduced into proliferating cells in vivo (e.g., bone marrow) or in vitro (e.g., fibroblast cultures) and the modified cells transferred to the site where the gene expression is required. Gene therapy may be particularly useful for treating enzyme deficiency diseases, hemoglobinopathies, and leukemias and may also prove useful in restoring drug sensitivity, particularly for leukemia.
The expression of a gene in an abnormal place, or at an abnormal time in an organism. Ectopic Gene Expression is often induced artificially by genetic techniques.
A form of gene interaction whereby the expression of one gene interferes with or masks the expression of a different gene or genes. Genes whose expression interferes with or masks the effects of other genes are said to be epistatic to the effected genes. Genes whose expression is affected (blocked or masked) are hypostatic to the interfering genes.
Techniques used to add in exogenous gene sequence such as mutated genes; REPORTER GENES, to study mechanisms of gene expression; or regulatory control sequences, to study effects of temporal changes to GENE EXPRESSION.
The number of copies of a given gene present in the cell of an organism. An increase in gene dosage (by GENE DUPLICATION for example) can result in higher levels of gene product formation. GENE DOSAGE COMPENSATION mechanisms result in adjustments to the level GENE EXPRESSION when there are changes or differences in gene dosage.
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