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Expression, purification and crystallization of an SLC16 monocarboxylate transporter family homologue specific for l-lactate.

08:00 EDT 9th September 2019 | BioPortfolio

Summary of "Expression, purification and crystallization of an SLC16 monocarboxylate transporter family homologue specific for l-lactate."

l-lactate plays an important role as metabolite and signaling molecule in eukaryotes and bacteria. Monocarboxylate transporters (MCTs) of the SLC16 solute carrier family are responsible for the transport of l-lactate across eukaryotic and bacterial cell membranes. Here we report an efficient protocol for the expression and purification of an SLC16 family homologue in milligram amounts. The purified protein is stable and can thus be used for biochemical and structural studies as shown by successful crystallization.

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This article was published in the following journal.

Name: Protein expression and purification
ISSN: 1096-0279
Pages: 105484

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Medical and Biotech [MESH] Definitions

An ATP binding cassette transporter, sub-family G protein that functions as a heterodimer with ATP BINDING CASSETTE TRANSPORTER, SUB-FAMILY G, MEMBER 8 in the selective transport of DIETARY CHOLESTEROL and PHYTOSTEROLS into and export out of ENTEROCYTES. It also functions in selective sterol excretion by the liver into bile.

An ATP binding cassette transporter, sub-family G protein that functions as a heterodimer with ATP BINDING CASSETTE TRANSPORTER, SUB-FAMILY G, MEMBER 5 in the selective transport of DIETARY CHOLESTEROL and PHYTOSTEROLS into and export out of ENTEROCYTES. It also functions in selective sterol excretion by the liver into bile.

An ATP binding cassette transporter, sub-family G protein that functions as a high capacity UREA exporter, transporter of STEROLS, and in the absorption and efflux of many drugs. Its efflux activity for ANTINEOPLASTIC AGENTS contributes to DRUG RESISTANCE. It functions as a homodimer and is expressed by cells in a variety of organs, as well as by NEOPLASTIC STEM CELLS.

A high-affinity, low capacity system y+ amino acid transporter with strong similarity to CATIONIC AMINO ACID TRANSPORTER 1. The two isoforms of the protein, CAT-2A and CAT-2B, exist due to alternative mRNA splicing. The transporter has specificity for the transport of ARGININE; LYSINE; and ORNITHINE.

A sub-family of ATP-binding cassette transporters that localize to the membranes of PEROXISOMES; ENDOPLASMIC RETICULUM; and LYSOSOMES. Members contain a transmembrane domain in their N-terminal half and generally function as homodimers in the transport of LIPIDS; BILE ACIDS; and VITAMIN B12. Mutations in some ABCD transporter genes are associated with PEROXISOMAL DISORDERS.

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