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Changes in the microbial community were investigated during the acclimation process of anaerobic digestion while treating synthetic lipid-rich wastewater, which comprised of glucose, acetic acid, lactic acid, and soybean oil. The oil content in the synthetic wastewater was increased successively from 0% to 25% and finally to 50% of the total carbon content, to clarify the effect of substrate type change from easily degradable organic materials to lipid. The oil decomposition-associated methane production rate increased as the microorganisms acclimated to the oil and eventually levelled off around 0.76 L/d. Analysis of the microbial community using next generation 16S rRNA gene sequencing (NGS) revealed the characteristic changes of dominant microorganisms Synergistales, Anaerolineales, Actinomycetales, and Nitrospirales from the domain bacteria, and Methanobacteriales and Methanosarcinales from the domain archaea. The increase in the relative abundance of Synergistales was found to be highly correlated with the increased rate of methane production from oil.
This article was published in the following journal.
Name: Journal of biotechnology
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The process of breakdown of food for metabolism and use by the body.
Community or individual involvement in the decision-making process.
The collective designation of three organizations with common membership: the European Economic Community (Common Market), the European Coal and Steel Community, and the European Atomic Energy Community (Euratom). It was known as the European Community until 1994. It is primarily an economic union with the principal objectives of free movement of goods, capital, and labor. Professional services, social, medical and paramedical, are subsumed under labor. The constituent countries are Austria, Belgium, Denmark, Finland, France, Germany, Greece, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, and the United Kingdom. (The World Almanac and Book of Facts 1997, p842)
Collaborative process of research involving researchers and community representatives.
Process by which unwanted microbial, plant or animal materials or organisms accumulate on man-made surfaces.
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. During DNA sequencing, the bases of a small fragment of DNA are sequentially identified from signals emitted as each fragment is re-synthesized from a ...