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Evaluation of the preventive and therapeutic effects of a recombinant vector co-expressing prostate-specific stem cell antigen (PSCA) and Clostridium perfringens enterotoxin (CPE) on prostate cancer in rats.

08:00 EDT 12th September 2019 | BioPortfolio

Summary of "Evaluation of the preventive and therapeutic effects of a recombinant vector co-expressing prostate-specific stem cell antigen (PSCA) and Clostridium perfringens enterotoxin (CPE) on prostate cancer in rats."

The effects of Clostridium perfringens (C.perfringens) enterotoxin (CPE) and prostate stem cell antigen (PSCA) on cancer prevention or treatment have been previously studied separately. For the first time, here we have elaborated a recombinant vector to co-express and study the cumulative effects of both of these factors on prostate cancer (PCa) in an animal model. The recombinant pBudCE4.1-cpe-PSCA vector was constructed in large scale. Rats were vaccinated by vector or vector plus chitosan nanoparticles before or after induction of PCa (preventive or therapeutic studies) by MNU and testosterone. Prostate tumors were weighed and histologically examined. Tumors and infusion site tissues as well as blood samples of all rats were collected and assessed by serological and molecular tests. We showed that vaccination with vector (along with or without nanoparticles) led to lower PCa incidence and tumor weight. The L-1β, IL6, and TNF-α serum levels and their gene expression accompanied by C-CAM1 gene expression in vaccinated groups were significantly higher than controls while no difference was seen in CK20 expression among all groups. Our findings showed that vector could effectively stimulate the immune system of rats to either prevent or suppress the PCa tumors. Adding chitosan nanoparticles did not affect the results significantly. This article is protected by copyright. All rights reserved.

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This article was published in the following journal.

Name: Biotechnology progress
ISSN: 1520-6033
Pages: e2906

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Medical and Biotech [MESH] Definitions

Proteins secreted by the prostate gland. The major secretory proteins from the human prostate gland include PROSTATE-SPECIFIC ANTIGEN, prostate-specific acid phosphatase, prostate-specific membrane antigen, and prostate-specific protein-94.

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