Nuclei deformation reveals pressure distributions in 3D cell clusters.

08:00 EDT 12th September 2019 | BioPortfolio

Summary of "Nuclei deformation reveals pressure distributions in 3D cell clusters."

Measuring pressures within complex multi-cellular environments is critical for studying mechanobiology as these forces trigger diverse biological responses, however, these studies are difficult as a deeply embedded yet well-calibrated probe is required. In this manuscript, we use endogenous cell nuclei as pressure sensors by introducing a fluorescent protein localized to the nucleus and confocal microscopy to measure the individual nuclear volumes in 3D multi-cellular aggregates. We calibrate this measurement of nuclear volume to pressure by quantifying the nuclear volume change as a function of osmotic pressure in isolated 2D culture. Using this technique, we find that in multicellular structures, the nuclear compressive mechanical stresses are on the order of MPa, increase with cell number in the cluster, and that the distribution of stresses is homogenous in spherical cell clusters, but highly asymmetric in oblong clusters. This approach may facilitate quantitative mechanical measurements in complex and extended biological structures both in vitro and in vivo.


Journal Details

This article was published in the following journal.

Name: PloS one
ISSN: 1932-6203
Pages: e0221753


DeepDyve research library

PubMed Articles [23074 Associated PubMed Articles listed on BioPortfolio]

Distributions of endocrine cell clusters during porcine pancreatic development.

Pancreatic islet xenotransplantation is a potential treatment for diabetes mellitus, and porcine pancreas may provide a readily available source of islets. Islets in juvenile pigs are smaller than tho...

Role of dynamic nuclear deformation on genomic architecture reorganization.

Higher-order genomic architecture varies according to cell type and changes dramatically during differentiation. One of the remarkable examples of spatial genomic reorganization is the rod photorecept...

Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entry.

Control of cell size requires molecular size sensors that are coupled to the cell cycle. Rod-shaped fission yeast cells divide at a threshold size partly due to Cdr2 kinase, which forms nodes at the m...

Enhanced deep-ultraviolet responsivity in aluminum-gallium oxide photodetectors via structure deformation by high-oxygen-pressure pulsed laser deposition.

Aluminum-gallium oxide (AGO) thin films with wide bandgaps of greater than 5.0 eV were grown using pulsed laser deposition. As evidenced by X-ray photoelectron spectroscopy, X-ray diffraction, and tra...

On-Chip Cell Incubator for Simultaneous Observation of Culture with and without Periodic Hydrostatic Pressure.

This paper proposes a microfluidic device which can perform simultaneous observation on cell growth with and without applying periodic hydrostatic pressure (Yokoyama et al. , , 427). The device is cal...

Clinical Trials [11468 Associated Clinical Trials listed on BioPortfolio]

Statistical Analysis of Pediatric Task Force Data Base

To define pediatric sex-specific blood pressure percentiles that were a function of both age and height. Also, to define the precise age at which blood pressure distributions for differen...

Change in Symptom Clusters in HNC Patients

Head and neck cancer (HNC) is the sixth most common malignancy worldwide, causing more than 379,000 deaths in 2015 notwithstanding the breakthrough in cancer screening and medical treatmen...

The Use of Myocardial Deformation Imaging

Myocardial deformation imaging allows analysis of myocardial viability in ischemic left ventricular dysfunction. This study will evaluate the predictive value of myocardial deformation ima...

Symptom Clusters in Cancer Patients and Their Caregivers- a Longitudinal Study

The complexity of cancer patients' symptoms and health problems constitutes considerable distress and burden for patients and their family caregivers. To help them manage the illness effec...

Usefulness of Myocardial Deformation Imaging for Trastuzumab-induced Cardiotoxicity

Trastuzumab prolongs survival in patients with human epidermal growth factor receptor type 2-positive breast cancer. Sequential left ventricular (LV) ejection fraction (EF) assessment has ...

Medical and Biotech [MESH] Definitions

Cell groups within the internal medullary lamina of the thalamus. They include a rostral division comprising the paracentral, central lateral, central dorsal, and central medial nuclei, and a caudal division composed of the centromedian and parafascicular nuclei.

The pressure within the CARDIAC ATRIUM. It can be measured directly by using a pressure catheter (see HEART CATHETERIZATION). It can be also estimated using various imaging techniques or other pressure readings such as PULMONARY CAPILLARY WEDGE PRESSURE (an estimate of left atrial pressure) and CENTRAL VENOUS PRESSURE (an estimate of right atrial pressure).

The in vitro formation of clusters consisting of a cell (usually a lymphocyte) surrounded by antigenic cells or antigen-bearing particles (usually erythrocytes, which may or may not be coated with antibody or antibody and complement). The rosette-forming cell may be an antibody-forming cell, a memory cell, a T-cell, a cell bearing surface cytophilic antibodies, or a monocyte possessing Fc receptors. Rosette formation can be used to identify specific populations of these cells.

The dorsal posterior subdivision of the diencephalon. The epithalamus is generally considered to include the habenular nuclei (HABENULA) and associated fiber bundles, the PINEAL BODY, and the epithelial roof of the third ventricle. The anterior and posterior paraventricular nuclei of the thalamus are included with the THALAMIC NUCLEI although they develop from the same pronuclear mass as the epithalamic nuclei and are sometimes considered part of the epithalamus.

Microscopic study of the spacial distribution pattern of CHROMATIN in CELL NUCLEI and CELL NUCLEOLI.

Quick Search

DeepDyve research library

Relevant Topics

Biological Therapy
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...

Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Searches Linking to this Article