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Skeletal muscle satellite cells (SC) play an important role in muscle repair following injury. The regulation of SC activity is governed by myogenic regulatory factors (MRF), including MyoD, Myf5, myogenin and MRF4. The mRNA expression of these MRF in humans following muscle damage has been predominately measured in whole muscle homogenate. Whether the temporal expression of MRF in whole muscle homogenate reflects SC specific expression of MRF remains largely unknown. Sixteen young men (23.1±1.0 yrs) performed 300 unilateral eccentric contractions (180 deg·s) of the knee extensors. Percutaneous muscle biopsies from the were taken prior to (Pre) and 48 h post-exercise. Fluorescence-activated cell sorting analysis was utilized to purify NCAM muscle SC from the whole muscle homogenate. Forty-eight hours post-eccentric exercise, MyoD, Myf5 and myogenin mRNA expression was increased in whole muscle homogenate (~1.4-, ~4.0-, ~1.7-fold, respectively, p<0.05) and in isolated SC (~19.3-, ~17.5-, ~58.9-fold, respectively, p<0.05). MRF4 mRNA expression was not increased 48h post-exercise in whole muscle homogenate (p>0.05) or in isolated SC (p>0.05). In conclusion, our results suggest that the directional changes in mRNA expression of the MRF in whole muscle homogenate in response to acute eccentric exercise reflects that observed in isolated muscle SC.
This article was published in the following journal.
Name: Journal of applied physiology (Bethesda, Md. : 1985)
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A myogenic regulatory factor that controls myogenesis. Though it is not clear how its function differs from the other myogenic regulatory factors, MyoD appears to be related to fusion and terminal differentiation of the muscle cell.
A myogenic regulatory factor that controls myogenesis. Myogenin is induced during differentiation of every skeletal muscle cell line that has been investigated, in contrast to the other myogenic regulatory factors that only appear in certain cell types.
A family of muscle-specific transcription factors which bind to DNA in control regions and thus regulate myogenesis. All members of this family contain a conserved helix-loop-helix motif which is homologous to the myc family proteins. These factors are only found in skeletal muscle. Members include the myoD protein (MYOD PROTEIN); MYOGENIN; myf-5, and myf-6 (also called MRF4 or herculin).
A SKELETAL MUSCLE-specific transcription factor that contains a basic HELIX-LOOP-HELIX MOTIF. It plays an essential role in MUSCLE DEVELOPMENT.
A regulatory factor X transcription factor that is required for the expression of MHC CLASS II GENES. It also binds to inverted repeats of HEPATITIS B VIRUS DNA and is required for viral gene expression. RFX1 functions as a monomer or heterodimer with other RFX transcription factors.
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