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The Enhancement of Metal-Binding Properties in Hemoglobin: The Role of Mild Damaging Factors.

08:00 EDT 12th September 2019 | BioPortfolio

Summary of "The Enhancement of Metal-Binding Properties in Hemoglobin: The Role of Mild Damaging Factors."

X-ray studies revealed the considerable enhancement of metal-binding properties in human hemoglobin under exposure to mild damaging factors (in the presence of 0.09 M urea or upon heating for 30 min at 50C). Changes in the element composition of hemoglobin monolayer, formed on the water subphase in the Langmuir trough, have been monitored in real time by the total external reflection X-ray fluorescence measurements. X-ray absorption spectroscopy has been applied to study the local environment of zinc ions bound on hemoglobin molecules. According to these data each zinc ion is coordinated by four ligands, two of which are cysteine and histidine. The oxidative stress has been found to accelerate extensively the enhancement of metal- binding ability in protein. Two-stage mechanism has been proposed as a possible explanation of the observed phenomenon: first, in presence of the mild damaging agents protein molecules can undergo transition from native conformation to more labile intermediate state that increases the accessibility of amino acid residues (in particular cysteine). At the second stage, oxidation of cysteine and the subsequent activation of cysteine SH-groups can affect markedly the protein-metal interaction. The presented investigations provide a deeper insight into the pathogenesis of metabolic disorders that excessive concentrations of the endogenic toxicants might trigger in an organism.

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Name: The journal of physical chemistry. B
ISSN: 1520-5207
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