Protein-lipid interactions stabilise the oligomeric state of Bor1p from Saccharomyces cerevisiae.

08:00 EDT 12th September 2019 | BioPortfolio

Summary of "Protein-lipid interactions stabilise the oligomeric state of Bor1p from Saccharomyces cerevisiae."

The BOR proteins are integral membrane transporters which mediate efflux of boron. Structural studies for two BOR family members from Arabidopsis thaliana and Saccharomyces mikitiae indicate that the proteins exist as dimers. However, it remains unclear whether dimer formation is dependent on protein-lipid interactions or whether the dimer is the functional form of the protein. Here, we used the BOR1p protein from Saccharomyces cerevisiae (ScBOR1p), recombinantly expressed in its native host, to explore these aspects of BOR transporter structure and function. Native mass spectrometry (MS) revealed that ScBOR1p isolates as a monomer in a range of detergents. Lipidomics analysis showed that ScBOR1p co-isolates with phosphatidylserine (PS), phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI). Delipidation of ScBOR1p followed by addition of PS or PE causes formation of ScBOR1p dimers, while PI binds to the protein but doesn't form dimers does not drive formation of ScBOR1p dimers. Using a homology model of ScBOR1p, based on the known dimeric BOR structures, we identified a possible lipid binding site at the dimer interface comprising residues Arg265, Arg267, Arg480 and Arg481. A quadruple 4R/A mutant was expressed and isolated and also shown to be monomeric by native MS. However, addition of PS or PE to this mutant did not reform the dimer. Functional complementation analysis revealed that the 4R/A mutant had boron efflux activity. Taken together these data strongly indicate that the physiological form of the ScBOR1p is the dimer and that dimer formation is dependent on association with membrane lipids. In addition, the functional unit of ScBOR1p is the monomer.


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This article was published in the following journal.

Name: Analytical chemistry
ISSN: 1520-6882


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