Anti-Proliferative Ability and Fluorescence Tracking of Alpha-Linolenic Acid-Loaded Microemulsion as Label-Free Delivery Carriers in MDA-MB-231 Cells.

08:00 EDT 12th September 2019 | BioPortfolio

Summary of "Anti-Proliferative Ability and Fluorescence Tracking of Alpha-Linolenic Acid-Loaded Microemulsion as Label-Free Delivery Carriers in MDA-MB-231 Cells."

In this work, the effects of alpha-linolenic acid (ALA) loaded in oil-in-water (O/W) and water-in-oil-in-water (W/O/W) microemulsion on cell viability, lactic dehydrogenase (LDH) viability and reactive oxygen species (ROS) levels were examined using cell counting kit-8 (CCK-8), LDH assay kit and fluorescence microscope, respectively. The CCK-8 assay demonstrated that ALA inhibited MDA-MB-231 human breast cancer cells proliferation in a dose-dependent manner. Further, the results of LDH activity and ROS levels revealed that ALA-induced cancer cell damage was closely related to oxidative stress. Under the irradiation of ultraviolet light, the microemulsion without added fluorescent dye would emit bright blue fluorescence, and the fluorescent images of the cells treated with ALA-loaded O/W and W/O/W microemulsion at different incubation times were taken, which exhibited long-term photostability and biocompatibility. In addition, the fluorescence mechanism of the microemulsion was explained by immobilizing surfactant molecules with aggregation-induced emission (AIE) properties at the water-oil interface through the microemulsion with a self-assembled structure. These findings showed the potential application of O/W and W/O/W microemulsion as the label-free delivery carriers in long-term imaging of living cells and real-time release monitoring of nutrients.


Journal Details

This article was published in the following journal.

Name: Journal of agricultural and food chemistry
ISSN: 1520-5118


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Medical and Biotech [MESH] Definitions

An omega-6 fatty acid produced in the body as the delta 6-desaturase metabolite of linoleic acid. It is converted to dihomo-gamma-linolenic acid, a biosynthetic precursor of monoenoic prostaglandins such as PGE1. (From Merck Index, 11th ed)

A fatty acid that is found in plants and involved in the formation of prostaglandins.

A plant genus of the family LAMIACEAE that is a source of perilla alcohol and the oil is rich in alpha-linolenic acid (alpha-18:3).

Eighteen-carbon cyclopentyl polyunsaturated fatty acids derived from ALPHA-LINOLENIC ACID via an oxidative pathway analogous to the EICOSANOIDS in animals. Biosynthesis is inhibited by SALICYLATES. A key member, jasmonic acid of PLANTS, plays a similar role to ARACHIDONIC ACID in animals.

(9 alpha,11 alpha,13E,15S)-9,11,15-Trihydroxyprost-13-en-1-oic acid (PGF(1 alpha)); (5Z,9 alpha,11,alpha,13E,15S)-9,11,15-trihydroxyprosta-5,13-dien-1-oic acid (PGF(2 alpha)); (5Z,9 alpha,11 alpha,13E,15S,17Z)-9,11,15-trihydroxyprosta-5,13,17-trien-1-oic acid (PGF(3 alpha)). A family of prostaglandins that includes three of the six naturally occurring prostaglandins. All naturally occurring PGF have an alpha configuration at the 9-carbon position. They stimulate uterine and bronchial smooth muscle and are often used as oxytocics.

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