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Inorganic carbon is an important carbon source of autotrophic bacteria, e.g., ammonia-oxidizing bacteria. Ammonia-oxidizing bacteria are chemoautotrophic bacteria with carbon sequestration capacity. Experiments were performed on partial nitrification sludge with different influent matrices, and optimal experimental operational conditions were established. The carbon fixation pathway of ammonia-oxidizing sludge was determined via C isotope tracers and qPCR. The denitrification effect was better when the NH-N, HCO, Ca, Mg, and microbial accelerant concentrations were 15, 250, 113, 100 and 1 mL/L, respectively. The nitrite accumulation rate reached 96.95%. C isotope tracing showed that C abundance in sludge increased significantly. The results showed that IC added into the influent participated in the carbon metabolism of microorganisms. The functional gene cbbL, which follows the Calvin cycle carbon sequestration pathway, was identified in the ammonia-oxidizing bacteria, and the effect of influent NH-N on the gene abundance was greater than that of other substrates.
This article was published in the following journal.
Name: Bioresource technology
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Any of several processes for the permanent or long-term artificial or natural capture or removal and storage of carbon dioxide and other forms of carbon, through biological, chemical or physical processes, in a manner that prevents it from being released into the atmosphere.
Unsaturated derivatives of the ESTRANES with methyl groups at carbon-13, with no carbon at carbon-10, and with no more than one carbon at carbon-17. They must contain one or more double bonds.
Enzymes that catalyze the joining of two molecules by the formation of a carbon-carbon bond. These are the carboxylating enzymes and are mostly biotinyl-proteins. EC 6.4.
Enzymes that catalyze the cleavage of a carbon-carbon bond by means other than hydrolysis or oxidation. This subclass contains the DECARBOXYLASES, the ALDEHYDE-LYASES, and the OXO-ACID-LYASES. EC 4.1.
Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...