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Genetically modified (GM) Atlantic salmon, AquAdvantage (AquAd), was the first GM animal approved officially for human consumption. Many countries monitor the use of this product under their GM regulations, but a pragmatic system for AquAd-specific detection is needed. Here, we developed a real-time polymerase chain reaction method with high sensitivity for detection of AquAd in foods. This method showed high specificity for the AquAd transgene and the detection limit was 12.5-25 targeted DNA copies per test reaction. An inter-laboratory study using the method developed demonstrated reproducibility at >0.1% (w/w) AquAd content.
This article was published in the following journal.
Name: Food chemistry
This research is aimed to establish a DPO-PCR method for rapid detection of wheat component in foods. A pair of highly specific DPO primers was designed using the wheat housekeeping gene GAG56D as the...
Duck adenovirus 3 (DAdV-3) is a newly identified duck adenovirus that has recently emerged in China. The incidence of duck infection caused by this virus is very high, with very large economic losses ...
In this work, a sensing nanochannel based on a N-[3-(triethoxysilyl)propyl]-4,5-dihydroimidazole (DHI) modified nanopipette was prepared and characterized for the ultrasensitive detection of cobalt io...
Quantitative real-time PCR (qPCR) is a widely adopted technique used for scientific, clinical, diagnostic, or quality control purposes. One of the main applications of qPCR is gene expression analysis...
Pyrophosphate-modified carbon quantum dots (PP-CDs) are demonstrated to be a viable fluorescent nanoprobe for mercury(II) (Hg) detection. Hg reacts with the pyrophosphate groups on the surface of PP-C...
This Phase I/II dose-escalation trial is designed to evaluate the safety and biological efficacy of allogeneic CMV, EBV and Adenovirus (tri-virus) specific cytotoxic T-lymphocytes (CTL) ge...
In this study the effects of genetically modified potatoes on the human metabolism will be observed. Healthy volunteers receive for one week muffins, produced with starch from a geneticall...
Starches from genetically modified potatoes, which differed in terms of amylose, amylopectin and phosphate content, were tested in a human randomized controlled trial.
This phase I trial studies the side effects of genetically modified T cells and decitabine in treating patients with ovarian, primary peritoneal, or fallopian tube cancer that has come bac...
This phase I/II trial studies the side effects and best dose of genetically modified T-cell therapy in treating patients with receptor tyrosine kinase-like orphan receptor 1 positive (ROR1...
Food derived from genetically modified organisms (ORGANISMS, GENETICALLY MODIFIED).
A method of detection of the number of cells in a sample secreting a specific molecule. Wtih this method a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.
Sensitive method for detection of bacterial endotoxins and endotoxin-like substances that depends on the in vitro gelation of Limulus amebocyte lysate (LAL), prepared from the circulating blood (amebocytes) of the horseshoe crab, by the endotoxin or related compound. Used for detection of endotoxin in body fluids and parenteral pharmaceuticals.
Method to determine the occurrence of OVULATION by direct or indirect means. Indirect methods examine the effects of PROGESTERONE on cervical mucus (CERVIX MUCUS), or basal body temperature. Direct ovulation detection, generally used in fertility treatment, involves analyses of circulating hormones in blood and ULTRASONOGRAPHY.
Polymerase Chain Reaction (PCR)
PCR (Polymerase Chain Reaction) uses the ability of DNA polymerase (enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two ident...