Photoactivation of silicon rhodamines via a light-induced protonation.

08:00 EDT 8th October 2019 | BioPortfolio

Summary of "Photoactivation of silicon rhodamines via a light-induced protonation."

Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore's outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.


Journal Details

This article was published in the following journal.

Name: Nature communications
ISSN: 2041-1723
Pages: 4580


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Medical and Biotech [MESH] Definitions

A trace element that constitutes about 27.6% of the earth's crust in the form of SILICON DIOXIDE. It does not occur free in nature. Silicon has the atomic symbol Si, atomic number 14, and atomic weight 28.09.

Blue-light receptors that regulate a range of physiological responses in PLANTS. Examples include: PHOTOTROPISM, light-induced stomatal opening, and CHLOROPLAST movements in response to changes in light intensity.

Inorganic compounds that contain silicon as an integral part of the molecule.

Silicon polymers that contain alternate silicon and oxygen atoms in linear or cyclic molecular structures.

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