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A single regulator NrtR controls bacterial NAD homeostasis via its acetylation.

08:00 EDT 9th October 2019 | BioPortfolio

Summary of "A single regulator NrtR controls bacterial NAD homeostasis via its acetylation."

Nicotinamide adenine dinucleotide (NAD) is an indispensable cofactor in all domains of life, the homeostasis of which requires tight regulation. Here we report that a Nudix-related transcriptional factor, designated MsNrtR (MSMEG_3198), controls the pathway of NAD biosynthesis in , a non-tuberculosis . The integrated evidence and confirms that MsNrtR is an auto-repressor, and negatively controls the NAD biosynthetic pathway. Binding of MsNrtR cognate DNA is finely mapped, which can be disrupted by an ADP-ribose intermediate. Unexpectedly, we discover that the acetylation of MsNrtR at Lysine 134 participates in the homeostasis of intra-cellular NAD level in . Furthermore, we demonstrate that NrtR acetylation proceeds via the non-enzymatic acetyl-phosphate (AcP) route rather than the enzymatic Pat/CobB pathway. In addition, the acetylation of NrtR also occurs in its paralogs of Gram-positive bacterium and Gram-negative bacterium , suggesting a common mechanism of post-translational modification in the context of NAD homeostasis. Together, it represents a first paradigm for the recruitment of acetylated NrtR to regulate bacterial central NAD metabolism.

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Name: eLife
ISSN: 2050-084X
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