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Hsp70 and Hsp40 chaperones work synergistically in a wide range of biological processes including protein synthesis, membrane translocation, and folding. We used nuclear magnetic resonance spectroscopy to determine the solution structure and dynamic features of an Hsp40 in complex with an unfolded client protein. Atomic structures of the various binding sites in the client complexed to the binding domains of the Hsp40 reveal the recognition pattern. Hsp40 engages the client in a highly dynamic fashion using a multivalent binding mechanism that alters the folding properties of the client. Different Hsp40 family members have different numbers of client-binding sites with distinct sequence selectivity, providing additional mechanisms for activity regulation and function modification. Hsp70 binding to Hsp40 displaces the unfolded client. The activity of Hsp40 is altered in its complex with Hsp70, further regulating client binding and release.
This article was published in the following journal.
Name: Science (New York, N.Y.)
Molecular chaperones are crucial for cellular life to ensure that all proteins obtain their right fold and functionality. Many chaperones are promiscuously binding a wide spectrum of client proteins, ...
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A procedure in which the therapist refrains from directing the client, but instead reflects back to the client what the latter has said, sometimes restating the client's remark.
A nondirective psychotherapy approach originated by Carl Rogers. The goals of therapy are to promote the client’s congruence, self awareness, and self acceptance. This approach views the client as naturally directed toward self actualization, and only needing facilitative conditions in order to promote this tendency.
A group of antibiotics that contain 6-aminopenicillanic acid with a side chain attached to the 6-amino group. The penicillin nucleus is the chief structural requirement for biological activity. The side-chain structure determines many of the antibacterial and pharmacological characteristics. (Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1065)
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