Track topics on Twitter Track topics that are important to you
In this study, the novel tube-fixed-bed bioreactor which consist of TubeSpin bioreactor 50 tube and 0.44 g macrocarriers was developed as the scale-down model of fixed-bed bioreactor. The adherent Vero cell-based pseudorabies virus (PRV) production process was tested in this novel model. The Vero cells grow well in tube-fixed-bed bioreactor and the cell density reached 5.8 × 10 cells/mL after 7 days culture. The PRV production parameters (time of infection (TOI), multiplicity of infection (MOI) and harvest process) were optimized in tube-fixed-bed bioreactor. Then the optimized process (TOI=3 d, MOI=0.001 and multiple harvest process) was 25-fold scale-up to Xcell 1 L laboratory-scale fixed-bed bioreactor and 125-fold scale-up to Xcell 5 L fixed-bed bioreactor successfully. The total PRV virus harvest in Xcell 1 L bioreactor at 5 day post infection (dpi) was 10.25 log TCID which corresponds to 177,827 doses of vaccine. The total PRV virus harvest in Xcell 5 L bioreactor at 5 dpi was 11.13 log TCID which corresponded to 1,348,962 doses of vaccine. The growth curve, metabolism and PRV production profile of three scale bioreactors were comparable confirmed the feasibility and scalability of tube-fixed-bed bioreactor as scale-down model of fixed-bed bioreactor for virus production process development.
This article was published in the following journal.
Name: Journal of pharmaceutical sciences
Molecular adjuvants are vaccine delivery vehicle to increase specific antigens effectiveness. Herein, we concentrated on IgG Fc, an effective molecular adjuvant, to develop novel pseudorabies virus (P...
: Current influenza vaccines are mainly produced from viruses propagated in eggs, an established process that has been developed over 70 years. However, this technology presents some drawbacks and oth...
A live attenuated cereal-based classical swine fever (CSF) vaccine for oral application, a form of vaccine that farmers can use themselves, demonstrated the improvement of CSF control in backyard prod...
Resveratrol had shown various properties before, like immunomodulatory, anti-inflammatory and antiviral activities. Based on these properties, the present study was designed to evaluate the effects an...
The paper discusses the techniques which are currently implemented for vaccine production based on virus-like particles (VLPs). The factors which determine the characteristics of VLP monomers assembly...
The purpose of this research is to demonstrate immunologic equivalence of three consecutive production lots of the subunit influenza vaccine compared to egg-derived inactivated influenza v...
This study will test the safety, tolerability, and immunogenicity of VARIVAX manufactured with the 2007 commercial VZV bulk process when concomitantly administered with M-M-R II in healthy...
Hepatitis B Vaccine [Recombinant] is a well-established vaccine which has been used extensively, worldwide since its initial licensure in 1986. Hepatitis B vaccines:  induce protection ...
The purpose of this research is to study side effects and safety of a new H5N1 flu (bird flu) vaccine and to look at how well people's immune systems make antibodies to fight infection aft...
This study is designed to test lot consistency of three different manufacturing lots and to generate safety and immunogenicity data of the investigational vaccine administered via the ID r...
A live attenuated virus vaccine of chick embryo origin, used for routine immunization of children and for immunization of adolescents and adults who have not had mumps or been immunized with live mumps vaccine. Children are usually immunized with measles-mumps-rubella combination vaccine.
A live attenuated virus vaccine of chick embryo origin, used for routine immunization of children and for immunization of adolescents and adults who have not had measles or been immunized with live measles vaccine and have no serum antibodies against measles. Children are usually immunized with measles-mumps-rubella combination vaccine. (From Dorland, 28th ed)
Vaccines or candidate vaccines used to prevent PSEUDORABIES (Aujeszky's disease), a herpesvirus of swine and other animals.
A live, attenuated varicella virus vaccine used for immunization against chickenpox. It is recommended for children between the ages of 12 months and 13 years.
A live VACCINIA VIRUS vaccine of calf lymph or chick embryo origin, used for immunization against smallpox. It is now recommended only for laboratory workers exposed to smallpox virus. Certain countries continue to vaccinate those in the military service. Complications that result from smallpox vaccination include vaccinia, secondary bacterial infections, and encephalomyelitis. (Dorland, 28th ed)
Antiretroviral Therapy Clostridium Difficile Ebola HIV & AIDS Infectious Diseases Influenza Malaria Measles Sepsis Swine Flu Tropical Medicine Tuberculosis Infectious diseases are caused by pathogenic...