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Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM).

08:00 EDT 11th October 2019 | BioPortfolio

Summary of "Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM)."

Membrane microdomains or lipid rafts compartmentalize cellular processes by laterally organizing membrane components. Such sub-membrane structures were mainly described in eukaryotic cells, but, recently, also in bacteria. Here, the protein content of lipid rafts in Escherichia coli was explored by mass spectrometry analyses of Detergent Resistant Membranes (DRM). We report that at least three of the four E. coli flotillin homologous proteins were found to reside in DRM, along with 77 more proteins. Moreover, the proteomic data were validated by subcellular localization, using immunoblot assays and fluorescence microscopy of selected proteins. Our results confirm the existence of lipid raft-like microdomains in the inner membrane of E. coli and represent the first comprehensive profiling of proteins in these bacterial membrane platforms.

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This article was published in the following journal.

Name: PloS one
ISSN: 1932-6203
Pages: e0223794

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