chi-miR-143-3p Promotes Apoptosis of Mammary Gland Epithelial Cells from Dairy Goats by Targeting .

08:00 EDT 11th October 2019 | BioPortfolio

Summary of "chi-miR-143-3p Promotes Apoptosis of Mammary Gland Epithelial Cells from Dairy Goats by Targeting ."

The mammary gland is an important organ for lactation in dairy goats. Mammary gland development and lactation functions are primarily regulated by natural hormones and certain crucial regulatory factors. family-interacting protein 1 () can specifically bind to neural precursor cell-expressed, developmentally downregulated protein 4 () family members to participate in ubiquitination, which in turn regulates a range of biological processes in the body. However, the effects of Ndfip1 expression regulation at the post-transcriptional level on the development of mammary gland cells have not been previously reported. To study the regulation of at post-transcriptional level, the overexpression and interference vectors of were constructed, and co-transfected into the primary mammary gland epithelial cells cultured with miR-143 mimics and inhibitor. Dual luciferase reporter gene system, real-time quantitative polymerase chain reaction, western blotting, cholecystokinin octapeptide assays, and flow cytometry were used to identify their regulation and function. As a result, was targeted and regulated by miR-143, which influences the development of mammary gland epithelial cells in dairy goats cultured . This study will lay an experimental foundation for further understanding the functions of and miR-143.


Journal Details

This article was published in the following journal.

Name: DNA and cell biology
ISSN: 1557-7430


DeepDyve research library

PubMed Articles [19064 Associated PubMed Articles listed on BioPortfolio]

Invited review: Accelerating mammary gland involution after drying-off in dairy cattle.

Bovine mammary gland involution, as a part of the reproductive cycle in dairy cows, is a very important remodeling transformation of the mammary gland for the subsequent lactation. There is considerab...

Dry period heat stress induces microstructural changes in the lactating mammary gland.

The bovine dry period is a non-lactating period between consecutive lactations characterized by mammary gland involution and redevelopment phases to replace senescent mammary epithelial cells with act...

miR-142-3p Regulates Milk Synthesis and Structure of Murine Mammary Glands via PRLR-Mediated Multiple Signaling Pathways.

Murine mammary gland is an ideal model for studying the development and milk synthesis of dairy animals. MicroRNAs play an important role in milk synthesis and mammary gland development,however, the...

The genomic landscape of estrogen receptor α binding sites in mouse mammary gland.

Estrogen receptor α (ERα) is the major driving transcription factor in the mammary gland development as well as breast cancer initiation and progression. However, the genomic landscape of ERα bindi...

Transcription Factor Sox4 as a Potential Player in Mammary Gland Involution.

Mammary gland involution is a regressive process for the gland to return to its prepregnancy state after lactation and comprises an initial reversible and second remodeling stage. Although many genes ...

Clinical Trials [9150 Associated Clinical Trials listed on BioPortfolio]

MACSSI (MAstectomie Cellules Souches Surveillance Immunitaire)

Monocentric, observational and prospective study in adult women having an early-stage breast cancer without genetic risk. The main objective is to characterize quantitatively and qualitat...

Dairy Foods and Weight Loss

Obesity is a national epidemic with multiple causes and complex solutions. Research in both animals and humans has suggested that the inclusion of dairy foods into a moderate calorie rest...

Prevention of UV- Induced Apoptosis by Caffeine

Investigate if caffeine accumulation in human lens epithelial cells after oral caffeine intake is sufficient to prevent from experimental ultraviolet radiation induced apoptosis

Prenatal Administration of Spermine Promotes Maturation of Premature Fetal Gut Epithelial Tight Junction: Experimental Study on Fetal Rabbit

Infections, particularly on the gastrointestinal tract, has been known to be one of the leading causes of death in preterm infants. This is due to the immaturity of the intestinal epitheli...

The Impact of Low-fat and Full-fat Dairy Consumption on Glucose Homeostasis (DAIRY Study)

This randomized controlled feeding trial aims to determine whether the consumption of different amounts and types of dairy products affects blood sugar regulation and cardiometabolic healt...

Medical and Biotech [MESH] Definitions

Tumors or cancer of the MAMMARY GLAND in animals (MAMMARY GLANDS, ANIMAL).

A glandular epithelial cell or a unicellular gland. Goblet cells secrete MUCUS. They are scattered in the epithelial linings of many organs, especially the SMALL INTESTINE and the RESPIRATORY TRACT.

Epithelial cells of the THYROID GLAND that produce and secrete THYROXINE and TRIIODOTHYRONINE.

A flavoprotein that functions as a powerful antioxidant in the MITOCHONDRIA and promotes APOPTOSIS when released from the mitochondria. In mammalian cells AIF is released in response to pro-apoptotic protein members of the bcl-2 protein family. It translocates to the CELL NUCLEUS and binds DNA to stimulate CASPASE-independent CHROMATIN condensation.

A benign, slow-growing tumor, most commonly of the salivary gland, occurring as a small, painless, firm nodule, usually of the parotid gland, but also found in any major or accessory salivary gland anywhere in the oral cavity. It is most often seen in women in the fifth decade. Histologically, the tumor presents a variety of cells: cuboidal, columnar, and squamous cells, showing all forms of epithelial growth. (Dorland, 27th ed)

Quick Search

DeepDyve research library

Relevant Topic

Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Searches Linking to this Article