TALEN-based chemically inducible, dimerization-dependent, sequence-specific nucleases.

08:00 EDT 11th October 2019 | BioPortfolio

Summary of "TALEN-based chemically inducible, dimerization-dependent, sequence-specific nucleases."

For precise genome editing, it is important to control the activity of sequence-specific nucleases (SSNs). We have constructed a chemically inducible nuclease system based on the dimerization of FKBP and FRB domains in the presence of rapamycin, and designated as a chemically inducible dimerization (CID). The CID was designed to occur at the inter-linker section between DNA binding domains and the FokI catalytic domain. Thus, induction of cleavage should occur quickly after addition of rapamycin because components of proteins are already in active form and located in nucleus. This CID-dependent sequence-specific nuclease (CID-SSN) has potential to be applied for time-resolved analysis of mutation induction mechanism in the genome.


Journal Details

This article was published in the following journal.

Name: Biochemistry
ISSN: 1520-4995


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