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Simultaneous visualization and quantitation of dual antigens adsorbed on adjuvants using high content analysis.

08:00 EDT 11th October 2019 | BioPortfolio

Summary of "Simultaneous visualization and quantitation of dual antigens adsorbed on adjuvants using high content analysis."

Traditional antigenicity assay requires antigen recovery from the particulate adjuvants prior to analysis. An method was developed for interrogating vaccine antigens with monoclonal antibodies while being adsorbed on adjuvants. The fluorescence imaging-based high content analysis was used to visualize the antigen distribution on adjuvant agglomerates and to analyze the antigenicity for adsorbed antigens. Simultaneous visualization and quantitation were achieved for dual antigens in a bivalent human papillomavirus vaccine with uniquely labeled antibodies. Good agreement was observed between the multiplexed assays with well-established sandwich enzyme-linked immunosorbent assays. The streamlined procedures and the amenability for multiplexing make the antigenicity analysis a favorable choice for functional assessment of bionanoparticles as vaccine antigens.

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Name: Nanomedicine (London, England)
ISSN: 1748-6963
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Medical and Biotech [MESH] Definitions

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