Simultaneous visualization and quantitation of dual antigens adsorbed on adjuvants using high content analysis.

08:00 EDT 11th October 2019 | BioPortfolio

Summary of "Simultaneous visualization and quantitation of dual antigens adsorbed on adjuvants using high content analysis."

Traditional antigenicity assay requires antigen recovery from the particulate adjuvants prior to analysis. An method was developed for interrogating vaccine antigens with monoclonal antibodies while being adsorbed on adjuvants. The fluorescence imaging-based high content analysis was used to visualize the antigen distribution on adjuvant agglomerates and to analyze the antigenicity for adsorbed antigens. Simultaneous visualization and quantitation were achieved for dual antigens in a bivalent human papillomavirus vaccine with uniquely labeled antibodies. Good agreement was observed between the multiplexed assays with well-established sandwich enzyme-linked immunosorbent assays. The streamlined procedures and the amenability for multiplexing make the antigenicity analysis a favorable choice for functional assessment of bionanoparticles as vaccine antigens.


Journal Details

This article was published in the following journal.

Name: Nanomedicine (London, England)
ISSN: 1748-6963


DeepDyve research library

PubMed Articles [5775 Associated PubMed Articles listed on BioPortfolio]

Effect of aluminum adjuvant and preservatives on structural integrity and physicochemical stability profiles of three recombinant subunit rotavirus vaccine antigens.

A non-replicating rotavirus vaccine (NRRV) containing three recombinant fusion proteins adsorbed to aluminum adjuvant (Alhydrogel, AH) is currently in clinical trials. The compatibility and stability ...

Simultaneous use of natural adjuvants and cell penetrating peptides improves HCV NS3 antigen-specific immune responses.

Exploring the interactions between engineered nanomaterials and immune cells at 3D Nano-bio interfaces to discover potent Nano-adjuvants.

Engineered nanomaterials (ENMs) as adjuvants can potentiate the adaptive immune responses to antigens by activating immune cells in three dimensional (3D) matrixes of tissues. However, few reports exp...

Aptamer induced multi-colored AuNCs-WS "Turn on" FRET Nano platform for dual-color simultaneous detection of aflatoxin B and zearalenone.

Mycotoxins posit serious threats to human and animal health, and numerous efforts have been performed to detect the multiple toxins by a single diagnostic approach. For the first time, we synthesized ...

Imitation of nature: Bionic design in the study of particle adjuvants.

Vaccine adjuvants play an increasingly important role in the development of modern vaccines. Among of them, particle adjuvants have received wide attention due to their unique properties. In order to ...

Clinical Trials [2282 Associated Clinical Trials listed on BioPortfolio]

Efficacy and Immunomodulation Study of Simultaneous Human Papillomavirus/ Hepatitis B (HPV/HBV) Vaccination

Rationale: In march 2009 the Dutch Health Council advised to introduce general infant hepatitis B (HBV) vaccination in the Dutch national immunization program (NIP) {Gezondheidsraad 2009 ...

Effects of Brain Network by Simultaneous Dual-mode Stimulation in Subacute Stroke Patients

Repetitive transcranial magnetic stimulation (rTMS) or transcranial direct current stimulation (tDCS) has been used for the modulation of stroke patients' motor function by altering the co...

Confocal Microscopy Dual Band in the Management of Bladder Cancer

Bladder cancer is one of the most common cancer worldwide and the second most frequent urological cancer. The photodynamic diagnosis technique (PDD) currently used (Hexvix®) has improved ...

Study Evaluating Vaccine in Adults With HIV

The main purpose of this study is to learn whether the study vaccine and the adjuvants(drugs that are used to help immune response) have an acceptable safety profile in treating individual...

Innate Immunity Gene Polymorphisms and Yeast Colonization

It is proposed to carry out the study in three medical or surgical intensive care units (ICU) in the CHRU, Lille, and the CHU, Besançon. In all patients admitted to these ICU (see Figure ...

Medical and Biotech [MESH] Definitions

A phenomenon manifested by an agent or substance adhering to or being adsorbed on the surface of a red blood cell, as tuberculin can be adsorbed on red blood cells under certain conditions. (Stedman, 25th ed)

Passive agglutination tests in which antigen is adsorbed onto latex particles which then clump in the presence of antibody specific for the adsorbed antigen. (From Stedman, 26th ed)

Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.

X-ray visualization of the chest and organs of the thoracic cavity. It is not restricted to visualization of the lungs.

The simultaneous, or near simultaneous, transference of heart and lungs from one human or animal to another.

Quick Search

DeepDyve research library

Relevant Topics

An assay is an analytic procedure for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity.  This can be a drug or biochemical substance or a cell in an organism or organic sample. ...

A vaccine is any preparation intended to produce immunity to a disease by stimulating the production of antibodies. It creates immunity but does not cause the disease. There are several differnt types of vaccine avalable; Killed microorganisms; which s...

Biological Therapy
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...

Searches Linking to this Article