Identification and characterization of ethanol inducible promoters of Zymomonas mobilis based on omics data and dual reporter-gene system.

08:00 EDT 18th October 2019 | BioPortfolio

Summary of "Identification and characterization of ethanol inducible promoters of Zymomonas mobilis based on omics data and dual reporter-gene system."

Zymomonas mobilis is a model bacterial ethanologen, and has been engineered to produce lignocellulosic biofuels and biochemicals such as 2,3-butanediol. We have previously identified promoters of different strengths using systems biology datasets, and characterized them using the flow cytometry-based dual reporter-gene system. Here, we further demonstrated the capability of applying the dual reporter-gene system and omics datasets on discovering inducible promoters. Ten candidate ethanol inducible promoters were identified through omics datasets mining and clustering. Using the dual reporter-gene system, these promoters were characterized under natural growth, ethanol stress and ethanol induced condition to investigate the transcriptional strength and ethanol inducibility. The results demonstrated that three promoters of P0405, P0435 and P0038 driving the expression of native genes of ZMO0405, ZMO0435 and ZMO0038 correspondingly are potential ethanol responsive promoters, and may be growth-related. This study not only identified and verified three ethanol inducible promoters as biological parts, which can be used to synchronize the expression of heterologous pathway genes with ethanol production process of Z. mobilis, but also demonstrated the power of combining omics datasets and dual reporter-gene system to identify biological parts for metabolic engineering and synthetic biology applications in Z. mobilis and related microorganisms. This article is protected by copyright. All rights reserved.


Journal Details

This article was published in the following journal.

Name: Biotechnology and applied biochemistry
ISSN: 1470-8744


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