Topics

Identification and characterization of ethanol inducible promoters of Zymomonas mobilis based on omics data and dual reporter-gene system.

08:00 EDT 18th October 2019 | BioPortfolio

Summary of "Identification and characterization of ethanol inducible promoters of Zymomonas mobilis based on omics data and dual reporter-gene system."

Zymomonas mobilis is a model bacterial ethanologen, and has been engineered to produce lignocellulosic biofuels and biochemicals such as 2,3-butanediol. We have previously identified promoters of different strengths using systems biology datasets, and characterized them using the flow cytometry-based dual reporter-gene system. Here, we further demonstrated the capability of applying the dual reporter-gene system and omics datasets on discovering inducible promoters. Ten candidate ethanol inducible promoters were identified through omics datasets mining and clustering. Using the dual reporter-gene system, these promoters were characterized under natural growth, ethanol stress and ethanol induced condition to investigate the transcriptional strength and ethanol inducibility. The results demonstrated that three promoters of P0405, P0435 and P0038 driving the expression of native genes of ZMO0405, ZMO0435 and ZMO0038 correspondingly are potential ethanol responsive promoters, and may be growth-related. This study not only identified and verified three ethanol inducible promoters as biological parts, which can be used to synchronize the expression of heterologous pathway genes with ethanol production process of Z. mobilis, but also demonstrated the power of combining omics datasets and dual reporter-gene system to identify biological parts for metabolic engineering and synthetic biology applications in Z. mobilis and related microorganisms. This article is protected by copyright. All rights reserved.

Affiliation

Journal Details

This article was published in the following journal.

Name: Biotechnology and applied biochemistry
ISSN: 1470-8744
Pages:

Links

DeepDyve research library

PubMed Articles [21844 Associated PubMed Articles listed on BioPortfolio]

Bioinformatic Identification of Chinese Hamster Ovary (CHO) Cold-shock Genes and Biological Evidence of their Cold-inducible Promoters.

Lower cultivation temperature dramatically affects cell growth and cellular productivity in CHO and is often used in industrial applications with the aim to enhance productivity. Cold-inducible protei...

iPromoter-2L2.0: Identifying Promoters and Their Types by Combining Smoothing Cutting Window Algorithm and Sequence-Based Features.

Promoters are short regions at specific locations of DNA sequences, which are playing key roles in directing gene transcription. They can be grouped into six types (σ,σ,σ,σ,σ,σ). Recently, a pre...

Identification of prokaryotic promoters and their strength by integrating heterogeneous features.

The promoter is a regulatory DNA region and important for gene transcriptional regulation. It is located near the transcription start site (TSS) upstream of the corresponding gene. In the post-genomic...

Long-term exposure to daily ethanol injections in DBA/2J and Swiss mice: Lessons for the interpretation of ethanol sensitization.

Most mice ethanol sensitization studies focused on neurobiology at the expense of its behavioral characterization. Furthermore, relatively short ethanol exposures (10 to 20 injections) were used in th...

Sensing response characterization of a micro-holographic sensor and its kinetics simulation.

A photopolymer-based micro-holographic sensor was developed to improve the sensitivity in sensing environmental factors. The micro-holographic grating can be formed by interference of two counter-prop...

Clinical Trials [5401 Associated Clinical Trials listed on BioPortfolio]

Systemic and Local Diffusion of Ethanol After Administration of Ethanol 96% Formulated in a Gel and Ethanol 98% Solution by the Percutaneous Route, in Patients With Congenital Venous Malformations:Pharmacokinetic, Pharmacodynamic and Clinical Study.

Absolute ethanol has been used "off-label" as an unmodified formulation (solution) in CVM. Despite its effectiveness, absolute ethanol appears difficult to handle because of its high diffu...

The Effects of Ethanol on Intestinal Permeability and Gut Hormones

The aime of this study is to envsitigate if ethanol has an incretin effect. This is done by administration of ethanol orally vs. an isoethanolaemic i.v. infusion of ethanol.

The Impact of Vaping Ethanol in the Evaluation of Impairment

The purpose of this research study is to find out about ethanol-containing e-cigarettes impact ethanol breath tests, field sobriety tests, or other tests of sobriety. Ethanol is a common p...

Substrate Metabolism During Exercise Following Ingestion of Ethanol

This study will investigate how ethanol (pure alcohol) influences carbohydrate and fat metabolism during prolonged, moderate intensity exercise. Participants will perform two bouts of cycl...

Endtidal Ethanol in Sclerotherapy

During the ethanol sclerotherapy procedure, the investigators will record doses of and time of ethanol injections and record ethanol concentrations in endtidal breath every 5 minutes. Furt...

Medical and Biotech [MESH] Definitions

A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that is not known to be pathogenic for man, animals, or plants. Its organisms are spoilers for beers and ciders and in sweet English ciders they are the causative agents of a secondary fermentation known as "cider sickness." The species Z. mobilis is used for experiments in molecular genetic studies.

An ethanol-inducible cytochrome P450 enzyme that metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Substrates include alcohol; NITROSAMINES; BENZENE; URETHANE; and other low molecular weight compounds. CYP2E1 has been used as an enzyme marker in the study of alcohol abuse.

A family of transcription factors that share an N-terminal HELIX-TURN-HELIX MOTIF and bind INTERFERON-inducible promoters to control GENE expression. IRF proteins bind specific DNA sequences such as interferon-stimulated response elements, interferon regulatory elements, and the interferon consensus sequence.

A B7 antigen that binds specifically to INDUCIBLE T-CELL CO-STIMULATOR PROTEIN on T-CELLS. It provides a costimulatory signal for T-cell proliferation and cytokine secretion.

A costimulatory receptor that is specific for INDUCIBLE T-CELL CO-STIMULATOR LIGAND. The receptor is associated with a diverse array of immunologically-related effects including the increased synthesis of INTERLEUKIN 10 in REGULATORY T-LYMPHOCYTES and the induction of PERIPHERAL TOLERANCE.

Quick Search


DeepDyve research library

Relevant Topics

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Stress
Stress is caused by your perception of situations around you and then the reaction of your body to them. The automatic stress response to unexpected events is known as 'fight or flight'. Discovered by Walter Cannon in 1932, it is the release of h...

Biological Therapy
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...


Searches Linking to this Article