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Zymomonas mobilis is a model bacterial ethanologen, and has been engineered to produce lignocellulosic biofuels and biochemicals such as 2,3-butanediol. We have previously identified promoters of different strengths using systems biology datasets, and characterized them using the flow cytometry-based dual reporter-gene system. Here, we further demonstrated the capability of applying the dual reporter-gene system and omics datasets on discovering inducible promoters. Ten candidate ethanol inducible promoters were identified through omics datasets mining and clustering. Using the dual reporter-gene system, these promoters were characterized under natural growth, ethanol stress and ethanol induced condition to investigate the transcriptional strength and ethanol inducibility. The results demonstrated that three promoters of P0405, P0435 and P0038 driving the expression of native genes of ZMO0405, ZMO0435 and ZMO0038 correspondingly are potential ethanol responsive promoters, and may be growth-related. This study not only identified and verified three ethanol inducible promoters as biological parts, which can be used to synchronize the expression of heterologous pathway genes with ethanol production process of Z. mobilis, but also demonstrated the power of combining omics datasets and dual reporter-gene system to identify biological parts for metabolic engineering and synthetic biology applications in Z. mobilis and related microorganisms. This article is protected by copyright. All rights reserved.
This article was published in the following journal.
Name: Biotechnology and applied biochemistry
Lower cultivation temperature dramatically affects cell growth and cellular productivity in CHO and is often used in industrial applications with the aim to enhance productivity. Cold-inducible protei...
Promoters are short regions at specific locations of DNA sequences, which are playing key roles in directing gene transcription. They can be grouped into six types (σ,σ,σ,σ,σ,σ). Recently, a pre...
The promoter is a regulatory DNA region and important for gene transcriptional regulation. It is located near the transcription start site (TSS) upstream of the corresponding gene. In the post-genomic...
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Systemic and Local Diffusion of Ethanol After Administration of Ethanol 96% Formulated in a Gel and Ethanol 98% Solution by the Percutaneous Route, in Patients With Congenital Venous Malformations:Pharmacokinetic, Pharmacodynamic and Clinical Study.
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A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that is not known to be pathogenic for man, animals, or plants. Its organisms are spoilers for beers and ciders and in sweet English ciders they are the causative agents of a secondary fermentation known as "cider sickness." The species Z. mobilis is used for experiments in molecular genetic studies.
An ethanol-inducible cytochrome P450 enzyme that metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Substrates include alcohol; NITROSAMINES; BENZENE; URETHANE; and other low molecular weight compounds. CYP2E1 has been used as an enzyme marker in the study of alcohol abuse.
A family of transcription factors that share an N-terminal HELIX-TURN-HELIX MOTIF and bind INTERFERON-inducible promoters to control GENE expression. IRF proteins bind specific DNA sequences such as interferon-stimulated response elements, interferon regulatory elements, and the interferon consensus sequence.
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A costimulatory receptor that is specific for INDUCIBLE T-CELL CO-STIMULATOR LIGAND. The receptor is associated with a diverse array of immunologically-related effects including the increased synthesis of INTERLEUKIN 10 in REGULATORY T-LYMPHOCYTES and the induction of PERIPHERAL TOLERANCE.
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