A Genomic Approach to Develop a New qPCR Test Enabling Detection of the Lineage Causing Wheat Blast.

08:00 EDT 23rd October 2019 | BioPortfolio

Summary of "A Genomic Approach to Develop a New qPCR Test Enabling Detection of the Lineage Causing Wheat Blast."

Rapid detection is key to managing emerging diseases because it allows their spread around the world to be monitored and limited. The first major wheat blast epidemics were reported in 1985 in the Brazilian state of Paraná. Following this outbreak, the disease quickly spread to neighboring regions and countries and, in 2016, the first report of wheat blast disease outside South America was released. This Asian outbreak was due to the trade of infected South American seed, demonstrating the importance of detection tests in order to avoid importing contaminated biological material into regions free from the pathogen. Genomic analysis has revealed that one particular lineage within the fungal species is associated with this disease: the lineage. A comparison of 81 genomes highlighted polymorphisms specific to the lineage, and this study developed a real-time PCR test targeting one of these polymorphisms. The test's performance was then evaluated in order to measure its analytical specificity, analytical sensitivity, and robustness. The C17 quantitative PCR test detected isolates belonging to the lineage with high sensitivity, down to 13 plasmid copies or 1 pg of genomic DNA per reaction tube. The blast-based approach developed here to study can be transposed to other emerging diseases.


Journal Details

This article was published in the following journal.

Name: Plant disease
ISSN: 0191-2917
Pages: PDIS04190685RE


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