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A colorimetric detection of microRNA-148a in gastric cancer by gold nanoparticles-RNA conjugates.

07:00 EST 8th November 2019 | BioPortfolio

Summary of "A colorimetric detection of microRNA-148a in gastric cancer by gold nanoparticles-RNA conjugates."

For the early diagnosis of gastric cancer, microRNA-148a as a promising biomarker is measured by a simple colorimetric biosensor due to the unique surface plasmon resonance (SPR) absorption of gold nanoparticles (AuNPs). In the assay system, the sensing probes are facilitated by the conjugation of AuNPs with RNA probes (RNAP) vis Au-S bond, which align in a tail-to-tail fashion onto the target RNA. When miRNA-148a is introduced, the sandwich hybridization reaction is triggered between the AuNPs-RNAP conjugates and targets, resulting in changes in SPR absorption band, microscopic distribution and macroscopic color of the AuNPs solution. Following the principle, this colorimetric method is able to quantitatively detect miRNA-148a at nanomolar level with a limit of ~1.9 nM and exhibits high sensitivity and selectivity by a low-cost UV-Vis spectrometer or even naked eye. Moreover, the AuNPs network materials with a characteristic sharp "melting transition" provide significant guidance for the reusability of DNA or RNA biosensor.

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Name: Nanotechnology
ISSN: 1361-6528
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