Topics

Extracellular Matrix Cues Modulate Schwann Cell Morphology, Proliferation, and Protein Expression.

07:00 EST 8th November 2019 | BioPortfolio

Summary of "Extracellular Matrix Cues Modulate Schwann Cell Morphology, Proliferation, and Protein Expression."

Peripheral nerve injuries require a complex set of signals from cells, macrophages, and the extracellular matrix (ECM) to induce regeneration across injury sites and achieve functional recovery. Schwann cells (SCs), the major glial cell in the peripheral nervous system (PNS), are critical to nerve regeneration due to their inherent capacity for altering phenotype post-injury to facilitate wound healing. The ECM plays a vital role in wound healing as well as regulating cell phenotype during tissue repair. To examine the underlying mechanisms between the ECM and SCs, this work sought to determine how specific ECM cues regulate the phenotype of SCs. To address this, SCs were cultured on polydimethylsiloxane substrates of a variable Young's modulus coated with ECM proteins. Cells were analyzed for spreading area, proliferation, cell and nuclear shape, and c-Jun expression. It was found that substrates with a stiffness of 8.67 kPa coated with laminin promoted the highest expression of c-Jun, a marker signifying a "regenerative" SC. Microcontact printed, cell adhesive areas were then utilized to precisely control the geometry and spreading of SCs and by controlling spreading area and cellular elongation, expression of c-Jun was either promoted or downregulated. These results begin to address the significant interplay between ECM cues and phenotype of SCs, while offering a potential means to enhance PNS regeneration through cellular therapies.

Affiliation

Journal Details

This article was published in the following journal.

Name: Journal of tissue engineering and regenerative medicine
ISSN: 1932-7005
Pages:

Links

DeepDyve research library

PubMed Articles [24552 Associated PubMed Articles listed on BioPortfolio]

Extracellular matrix-associated factors play critical roles in regulating pancreatic β-cell proliferation and survival.

The insulin-producing β cell is key for maintaining glucose homeostasis. The islet microenvironment greatly influences β-cell survival and proliferation. Within the islet, β cells contact the extra...

Surface Micro- and Nanoengineering: Applications of Layer-by-Layer Technology as a Versatile Tool to Control Cellular Behavior.

Extracellular matrix (ECM) cues have been widely investigated for their impact on cellular behavior. Among mechanics, physics, chemistry, and topography, different ECM properties have been discovered ...

Glycosaminoglycan Functionalization of Electrospun Scaffolds Enhances Schwann Cell activity.

Nerve fibers of the peripheral nervous system (PNS) have a remarkable ability to regenerate up to an almost complete recovery of normal function following a crush or a Sunderland Type II injury. This ...

Subcellular Control over Focal Adhesion Anisotropy, Independent of Cell Morphology, Dictates Stem Cell Fate.

Although microscale patterning techniques have been used to control cell morphology and shape, they only provide indirect control over the formation of the subcellular cytoskeletal elements that deter...

MiR-152-3p regulates cell proliferation, invasion and extracellular matrix expression through by targeting FOXF1 in keloid fibroblasts.

Emerging evidence has revealed that microRNAs (miRNAs) play critical roles in keloid pathogenesis. However, potential molecular mechanism of keloid formation remains unclear. In the present study, our...

Clinical Trials [9317 Associated Clinical Trials listed on BioPortfolio]

Role of Extracellular Matrix in the Development of Airway Remodeling in Asthma

Asthma is a major noncommunicable chronic inflammatory disorder which is characterized by airway inflammation and related to pathological modifications of the bronchial wall structure so c...

Autologous Human Schwann Cells in Peripheral Nerve Repair

The purpose of this study is to assess the safety of autologous human Schwann cell (ahSC) augmentation of nerve autograft repair in participants with severe peripheral nerve injury (PNI). ...

The Effect of Simvastatin on Uterine Leiomyoma Development and Growth in Infertile Women

Uterine leiomyomas with an estimated incidence of 50%-80% are the most common tumors of female reproductive system. Statins as a HMG-CoA reductase inhibitors inhibit the mevalonate pathway...

Endoscopic Mucosal Resection and Cellular Matrix

This study is being done to assess the use of scaffold products in promoting constructive healing as determined by the ability of the resection site to lift during follow-up.

Sputum Matrix Metalloproteinases (MMP) mRNA and Montelukast

Matrix metalloproteinases (MMPs) are a group of 24 zinc containing enzymes in man. These enzymes were originally described as cleaving extracellular matrix (ECM) substrates with a predo...

Medical and Biotech [MESH] Definitions

A discoidin domain receptor for FIBRILLAR COLLAGEN that functions in a variety of cellular processes. For example, it regulates cell attachment to the EXTRACELLULAR MATRIX, remodeling of the extracellular matrix, CELL MIGRATION; CELL DIFFERENTIATION; CELL PROLIFERATION; and CELL SURVIVAL.

A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.

A CCN protein family member found at high levels in NEPHROBLASTOMA cells. It is found both intracellularly and in the EXTRACELLULAR MATRIX and may play a role in the regulation of CELL PROLIFERATION and EXTRACELLULAR MATRIX synthesis.

An anchoring junction of the cell to a non-cellular substrate, similar in morphology to halves of DESMOSOMES. They are composed of specialized areas of the plasma membrane where INTERMEDIATE FILAMENTS bind on the cytoplasmic face to the transmembrane linkers, INTEGRINS, via intracellular attachment proteins, while the extracellular domain of the integrins binds to EXTRACELLULAR MATRIX PROTEINS.

A receptor for TWEAK cytokine that is highly expressed by cells in the heart, placenta, and kidney. It plays a role in ANGIOGENESIS and the proliferation of endothelial cells; it may also modulate cellular adhesion to the extracellular matrix.

Quick Search


DeepDyve research library

Relevant Topics

Nutrition
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Wound management
Anything that breaks the skin is a wound because when the skin is broken, there's a risk of germs getting into the body and causing an infection. Follow and track Wound Care News on BioPortfolio: Wound Car...


Searches Linking to this Article