Phosphate functionalization and enzymatic calcium mineralization synergistically enhance oligopoly(ethylene glycol) fumarate hydrogel osteoconductivity for bone tissue engineering.

07:00 EST 8th November 2019 | BioPortfolio

Summary of "Phosphate functionalization and enzymatic calcium mineralization synergistically enhance oligopoly(ethylene glycol) fumarate hydrogel osteoconductivity for bone tissue engineering."

A current approach in bone tissue engineering is the implantation of polymeric scaffolds that promote osteoblast attachment and growth as well as biomineralization. One promising polymer is oligo[poly(ethylene glycol) fumarate] (OPF), a polyethylene glycol (PEG)-based material that is biocompatible, injectable, and biodegradable, but in its native form doesn't support robust bone cell attachment or growth. To address this issue, this study evaluated the osteoconductivity of bis[2-(methacryloyloxy)ethyl] phosphate (BP) functionalized OPF hydrogels (OPF-BP) using MC3T3-E1 pre-osteoblast cells, both before and after enzymatic mineralization with a calcium solution. The inclusion of negatively charged functional groups allowed for the tailored uptake and release of calcium, while also altering the mechanical properties and surface topography of the hydrogel surface. In cell culture, OPF-BP hydrogels with 20% and 30% (w/w) BP optimized osteoblast attachment, proliferation, and differentiation after a 21-day in vitro period. In addition, the OPF-BP30 treatment, when mineralized with calcium, exhibited a 128% increase in osteocalcin expression when compared with the non-mineralized treatment. These findings suggest that phosphate functionalization and enzymatic calcium mineralization can act synergistically to enhance the osteoconductivity of OPF hydrogels, making this processed material an attractive candidate for bone tissue engineering applications. This article is protected by copyright. All rights reserved.


Journal Details

This article was published in the following journal.

Name: Journal of biomedical materials research. Part A
ISSN: 1552-4965


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A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.

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