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In this study, a halotolerant strain was isolated from high salinity leachate and identified as Bacillus cereus NT-3. It can produce high concentration of polyhydroxyalkanoates (PHAs) with no significant changes when NaCl concentration is up to 50 g/L. FTIR and NMR spectra of PHAs synthesized by Bacillus cereus NT-3 were similar to the standard or previous results. Effluent from acidogenic fermentation of food waste and pure volatile fatty acids (VFAs) mixture was used as carbon source to check the effect of non-VFAs compounds of the effluent on PHAs production. The maximum PHAs production was 0.42 g/L for effluent fermentation, while it was 0.34 g/L for pure VFAs fermentation, indicating that bacteria could use actual effluent in better way. Furthermore, a mathematical model was established for describing kinetic behavior of bacteria using different carbon source. These results provided a promising approach for PHAs biosynthesis with low-cost carbon source. This article is protected by copyright. All rights reserved.
This article was published in the following journal.
Name: Biotechnology and applied biochemistry
One of the advantages of microbial synthesis of polyhydroxyalkanoates (PHAs) is the production of diverse polymers with different properties by the addition of different monomers, such as 3-hydroxybut...
Ralstonia eutropha Re2133/pCB81 is able to utilize various volatile fatty acids (VFAs) (acetate, butyrate, lactate, and propionate) for polyhydroxyalkanoates (PHAs) production. Acetate and lactate res...
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Food is any substance consumed to provide nutritional support for the body. It is usually of plant or animal origin, and contains essential nutrients, such as carbohydrates, fats, proteins, vitamins, or minerals. The substance is ingested by an organism ...