Thermosensitive alternative splicing senses and mediates temperature adaptation in .

07:00 EST 8th November 2019 | BioPortfolio

Summary of "Thermosensitive alternative splicing senses and mediates temperature adaptation in ."

Circadian rhythms are generated by cyclic transcription, translation, and degradation of clock gene products, including (), but how the circadian clock senses and adapts to temperature changes is not completely understood. Here we show that temperature dramatically changes the splicing pattern of in . We found that at 18 °C, TIM levels are low due to the induction of two cold-specific isoforms: and . At 29 °C, another isoform, , is upregulated. This isoform switching regulates the levels and activity of TIM as each isoform has a specific function. We found that encodes a protein that rescues the behavioral defects of mutants and that flies in which is abrogated have abnormal locomotor activity. In addition, miRNA-mediated control limits the expression of some of these isoforms. Finally, our data using minigenes suggest that alternative splicing might act as a thermometer for the circadian clock.


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This article was published in the following journal.

Name: eLife
ISSN: 2050-084X


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Medical and Biotech [MESH] Definitions

A process whereby multiple RNA transcripts are generated from a single gene. Alternative splicing involves the splicing together of other possible sets of EXONS during the processing of some, but not all, transcripts of the gene. Thus a particular exon may be connected to any one of several alternative exons to form a mature RNA. The alternative forms of mature MESSENGER RNA produce PROTEIN ISOFORMS in which one part of the isoforms is common while the other parts are different.

An RNA splicing factor that performs a critical function in both constitutive and enhancer-dependent RNA SPLICING. It recruits RIBONUCLEOPROTEIN, U2 SMALL NUCLEAR to the splice site and mediates interactions between it, the RNA molecule, and other splicing factors for accurate 3'-splice site selection.

An RNA splicing factor that also binds DNA. It is essential for pre-mRNA spicing in the formation of early SPLICEOSOMES and catalytic step II. It binds specifically to polypyrimidine tracts in INTRONS and is involved in the regulation of ALTERNATIVE SPLICING by SIGNAL TRANSDUCTION PATHWAYS.

A family of regulatory factors essential for constitutive and alternative splicing in RNA metabolism.

A heterogeneous-nuclear ribonucleoprotein that has specificity for AU-rich elements found in the 3'-region of mRNA and may play a role in RNA stability. Several isoforms of hnRNP D protein have been found to occur due to alternative mRNA splicing (RNA SPLICING).

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