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Circadian rhythms are generated by cyclic transcription, translation, and degradation of clock gene products, including (), but how the circadian clock senses and adapts to temperature changes is not completely understood. Here we show that temperature dramatically changes the splicing pattern of in . We found that at 18 °C, TIM levels are low due to the induction of two cold-specific isoforms: and . At 29 °C, another isoform, , is upregulated. This isoform switching regulates the levels and activity of TIM as each isoform has a specific function. We found that encodes a protein that rescues the behavioral defects of mutants and that flies in which is abrogated have abnormal locomotor activity. In addition, miRNA-mediated control limits the expression of some of these isoforms. Finally, our data using minigenes suggest that alternative splicing might act as a thermometer for the circadian clock.
This article was published in the following journal.
The circadian pacemaker consists of transcriptional feedback loops subjected to post-transcriptional and post-translational regulation. While post-translational regulatory mechanisms have been studie...
The serine/arginine-rich (SR) proteins, have an essential role for the splicing of pre-mRNA in eukaryote. Pre-mRNA with introns can be alternative spliced to generate multiple transcripts, thereby inc...
Flowering transition is regulated by complex genetic networks in response to endogenous and environmental signals. Pre-mRNA splicing is an essential step for the post-transcriptional regulation of gen...
Cold shock proteins are thought to participate in the cold-tolerant nature of hibernating animals. We previously demonstrated that an alternative splicing may allow rapid induction of functional cold-...
Alternative splicing is a prevalent mechanism of gene regulation that is modulated in response to a wide range of extracellular stimuli. Stress-activated protein kinases (SAPKs) play a key role in con...
Aberrant RNA splicing and mutations in spliceosome complex in acute myeloid leukaemia (AML) are frequent. It have been shown that some splicing variants had a prognostic value in AML. AML...
- Working Hypothesis: EGCG and Tocotrienol can act as genetic modifiers and increase the level of correctly spliced CFTR transcripts. - Aims of the Study: To determine in patient...
This study is designed to test whether SCN5A mRNA processing is altered in OSA patients, which may contribute to their increased arrhythmic risk, and whether processing of SCN5A mRNA is mo...
The purpose of this pilot study is to determine how the anti-platelet drug, ticagrelor, impacts platelet mRNA splicing after a single loading dose in 10 healthy participants. These results...
Data from evaluating prostate cancer (PCa) biopsy tissue from AA and white patients has led to the discovery of alternative splicing as a novel molecular mechanism underlying more aggressi...
A process whereby multiple RNA transcripts are generated from a single gene. Alternative splicing involves the splicing together of other possible sets of EXONS during the processing of some, but not all, transcripts of the gene. Thus a particular exon may be connected to any one of several alternative exons to form a mature RNA. The alternative forms of mature MESSENGER RNA produce PROTEIN ISOFORMS in which one part of the isoforms is common while the other parts are different.
An RNA splicing factor that performs a critical function in both constitutive and enhancer-dependent RNA SPLICING. It recruits RIBONUCLEOPROTEIN, U2 SMALL NUCLEAR to the splice site and mediates interactions between it, the RNA molecule, and other splicing factors for accurate 3'-splice site selection.
An RNA splicing factor that also binds DNA. It is essential for pre-mRNA spicing in the formation of early SPLICEOSOMES and catalytic step II. It binds specifically to polypyrimidine tracts in INTRONS and is involved in the regulation of ALTERNATIVE SPLICING by SIGNAL TRANSDUCTION PATHWAYS.
A family of regulatory factors essential for constitutive and alternative splicing in RNA metabolism.
A heterogeneous-nuclear ribonucleoprotein that has specificity for AU-rich elements found in the 3'-region of mRNA and may play a role in RNA stability. Several isoforms of hnRNP D protein have been found to occur due to alternative mRNA splicing (RNA SPLICING).
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...
A microRNA (abbreviated miRNA) is a small non-coding RNA molecule (containing about 22 nucleotides) found in plants, animals, and some viruses. Key findings: miRNA is involved in the normal functioning of eukaryotic cells, so has dysregulation...