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Two novel strains of members of the genus , WCHEs120002 and WCHEs120003, were recovered from the sputum of two patients at a hospital in PR China in 2017. The strains were Gram-stain-negative, facultatively anaerobic, motile and non-spore-forming. The two strains were subjected to whole-genome sequencing. Phylogenetic analysis based on core genes of type strains of species of the family revealed that the two strains belonged to the genus r but were distinct from any previously known species of the genus. Both average nucleotide identity and DNA-DNA hybridization values between strains WCHEs120002 and WCHEs120003 and type strains of all known species of the genus were lower than the recommended thresholds of 95 and 70 %, respectively, for species delineation. The major fatty acids of the two strains were C, C cyclo and Cω7, which are similar to those of other species of the genus . Genomic DNA G+C contents of strains WCHEs120002 and WCHEs120003 were 56.09 and 55.91 mol%, respectively. WCHEs120002 ferments melibiose and sucrose but is negative for d-sorbitol and methyl-α-d-mannopyranoside reactions, which distinguish it from all other species of the genus . WCHEs120003 can be differentiated from other species of the genus by its ability to ferment potassium gluconate and its negative reactions for d-sorbitol and l-fucose. Genotypic and phenotypic characteristics indicate that strains WCHEs120002 and WCHEs120003 represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed, respectively. The type strain of sp. nov. is WCHEs120002 (=GDMCC1.1569=NCTC 14273) and the type strain of sp. nov. is WCHEs120003 (=GDMCC1.1568=NCTC 14274).
This article was published in the following journal.
Name: International journal of systematic and evolutionary microbiology
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