Topics

Arabidopsis PAD4 lipase-like domain is sufficient for resistance to green peach aphid.

07:00 EST 8th November 2019 | BioPortfolio

Summary of "Arabidopsis PAD4 lipase-like domain is sufficient for resistance to green peach aphid."

Plants have evolved mechanisms to protect themselves against pathogenic microbes and insect pests. In Arabidopsis, the immune regulator PAD4 functions with its cognate partner EDS1 to limit pathogen growth. PAD4, independently of EDS1, reduces infestation by green peach aphid (GPA). How PAD4 regulates these defense outputs is unclear. By expressing the N-terminal PAD4 lipase-like domain (PAD4) without its C-terminal 'EDS1-PAD4' (EP) domain, we interrogated PAD4 functions in plant defense. Here we show that transgenic expression of PAD4 in Arabidopsis is sufficient for limiting GPA infestation, but not for conferring basal and effector-triggered pathogen immunity. This suggests that the C-terminal PAD4 EP domain is necessary for EDS1-dependent immune functions. Moreover, PAD is not sufficient to interact with EDS1, indicating the PAD4-EP domain is required for stable heterodimerisation. These data provide molecular evidence that PAD4 has domain-specific functions.

Affiliation

Journal Details

This article was published in the following journal.

Name: Molecular plant-microbe interactions : MPMI
ISSN: 0894-0282
Pages:

Links

DeepDyve research library

PubMed Articles [11792 Associated PubMed Articles listed on BioPortfolio]

Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.

In plant innate immunity, enhanced disease susceptibility 1 (EDS1) integrates all pathogen-induced signals transmitted by TIR-type NLR receptors. Driven by an N-terminal α/β-hydrolase-folded domain ...

Functional characterization of chloroplast transit peptide in the small subunit of Rubisco in maize.

Functions of domains or motifs, which are encoded by the transit peptide (TP) of the precursor of the small subunit of Rubisco (prSSU), have been investigated intensively in dicots. Functional charact...

RPW8/HR repeats control NLR activation in Arabidopsis thaliana.

In many plant species, conflicts between divergent elements of the immune system, especially nucleotide-binding oligomerization domain-like receptors (NLR), can lead to hybrid necrosis. Here, we repor...

Constitutive expression of NtabSPL6-1 in tobacco and Arabidopsis could change the structure of leaves and promote the development of trichomes.

The coding sequence of NtabSPL6-1 was cloned by high-fidelity PCR with specific primers and was used in construction of a binary vector for overexpression. Wild-type Col-0 Arabidopsis plants and Qinya...

Cryptic chlorophyll breakdown in non-senescent green Arabidopsis thaliana leaves.

Chlorophyll (Chl) breakdown is a diagnostic visual process of leaf senescence, which furnishes phyllobilins (PBs) by the PAO/phyllobilin pathway. As Chl breakdown disables photosynthesis, it appears t...

Clinical Trials [3820 Associated Clinical Trials listed on BioPortfolio]

Efficacy and Safety of Human Lipoprotein Lipase (LPL)[S447X] Expressed by an Adeno-Associated Viral Vector in LPL-deficient Subjects

This trial is designed to expand the currently available data on the safety and efficacy of alipogene tiparvovec treatment in lipoprotein lipase deficiency (LPLD) and to further the unders...

Green Tea Anticancer Mechanisms in Smokers

The purpose of this study is to determine whether green tea may lower the risk of certain cancers.

Lysosomal Acid Lipase Increase After Chetogenic Diet.

The very low carbohydrates diet (VLCKD) induces liver steatosis amelioration. Lysosomal acid lipase (LAL) deficiency plays a role in fats accumulation in liver. To date, no studies have as...

GENIALL Lipoprotein Lipase Deficiency (LPLD) Disease Registry

Lipoprotein lipase deficiency (LPLD) is a rare autosomal recessive disorder, characterized by loss-of function mutations in the LPL gene, leading to the inability to produce functionally a...

Screening for Lysosomal Acid Lipase Deficiency

The primary outcome of this study is the development of a clinical profile of pediatric patients with LAL-D, which will enable the Sponsor to provide more focused guidance to the medical c...

Medical and Biotech [MESH] Definitions

A plant homeotic protein involved in the development of stamens and carpels of Arabidopsis thaliana. It is a DNA-binding protein that contains the MADS-box domain. It is one of the four founder proteins that structurally define the superfamily of MADS DOMAIN PROTEINS.

Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.

A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.

Compounds that increase the enzymatic activity of LIPOPROTEIN LIPASE. Lipoprotein lipase activators have a potential role in the treatment of OBESITY by increasing LIPID METABOLISM. Note that substances that increase the synthesis of lipoprotein lipase are not included here.

An inherited condition due to a deficiency of either LIPOPROTEIN LIPASE or APOLIPOPROTEIN C-II (a lipase-activating protein). The lack of lipase activities results in inability to remove CHYLOMICRONS and TRIGLYCERIDES from the blood which has a creamy top layer after standing.

Quick Search


DeepDyve research library

Relevant Topics

Collaborations in biotechnology
Commercial and academic collaborations are used throughout the biotechnology and pharmaceutical sector to enhance research and product development. Collaborations can take the form of research and evaluation agreements, licensing, partnerships etc. ...

Genetic Engineering
Genetic engineering is the process of manually adding new DNA to an organism. The goal is to add one or more new traits that are not already found in that organism. Examples of genetically engineered (transgenic) organisms currently on the market include...

Immunology
Allergies Automimmune Disease Human Papillomavirus (HPV) Immunology Vaccine Immunology is the study of immunity and the defence mechanisms of the body. A greater understanding of immunology is needed to develop vaccines, understand ...


Searches Linking to this Article