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Characterization of Isolates from Selected U.S. Swine Feed Mills by Whole-Genome Sequencing.

07:00 EST 8th November 2019 | BioPortfolio

Summary of "Characterization of Isolates from Selected U.S. Swine Feed Mills by Whole-Genome Sequencing."

Every year salmonellosis is responsible for $2.3 billion in costs to the U.S. food industry, with nearly 6% of the reported cases associated with pork and/or pork products. Several studies have demonstrated the role of pigs as reservoirs. Furthermore, this pathogen has been identified as a potential biological hazard in many livestock feeds. The overall objective of this research was to characterize isolates in selected U.S. swine feed mills by whole-genome sequencing (WGS) and evaluate isolates in association with the season and feed production stages. isolates were collected from 11 facilities during a previous study. Samples were analyzed for prevalence following the U.S. Department of Agriculture guidelines and confirmed by PCR. WGS was carried out on either the MiSeq or NextSeq sequencer. genome assemblies were obtained with the Shovill pipeline, version 0.9. ResFinder and SPIFinder were used to identify antibiotic resistance genes and pathogenicity islands. Finally, their phylogenetic relationship and diversity were determined by core genome multilocus sequence typing. Overall, our analysis showed the presence of in the feed mill environment. Isolates belonged to 16 different serotypes. Agona, Mbandaka, Senfenberg, and Scharzengrund were the most frequently found, and 18 single-nucleotide polymorphism clusters were identified. analysis showed that 40% of the strains carried at least one antimicrobial resistance gene. All isolates in this study could be considered of public health concern and pathogenic potential. Our findings underscore the potential role of the feed mill environment as the pathogen entry route into the human food value chain.

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This article was published in the following journal.

Name: Foodborne pathogens and disease
ISSN: 1556-7125
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