Stability-indicating HPLC method for acyclovir and lidocaine in topical formulations.

07:00 EST 22nd November 2019 | BioPortfolio

Summary of "Stability-indicating HPLC method for acyclovir and lidocaine in topical formulations."

A simple, rapid and accurate stability-indicating HPLC assay was developed for the determination of acyclovir and lidocaine in topical formulations. Chromatographic separation of acyclovir and lidocaine was achieved using a reversed-phase C column and a gradient mobile phase (20 mM ammonium acetate pH 3.5 in water and acetonitrile). The degradation products of acyclovir and lidocaine in the samples were analyzed by Ultra Performance Liquid Chromatography-Time of Flight Mass Spectrometry (UPLC-TOF-MS). The HPLC method successfully resolved the analytes from the impurities and degradation products in the topical formulation. Furthermore, the method detected the analytes from the human skin leachables following the extraction of the analytes in the skin homogenate samples. The method showed linearity over wide ranges of 5-500 μg/mL and 10-200 μg/mL for acyclovir and lidocaine in the topical product, respectively, with a correlation coefficient (r ) >0.9995. The relative standard deviation (% RSD) for precision, repeatability, and robustness of the method validation assays were less than 2%. The skin extraction efficiency for acyclovir and lidocaine was 92.8±0.7 and 91.3±3.2, respectively with no interference from the skin leachables. Thus, simultaneous quantification of acyclovir and lidocaine in the topical formulations was achieved.


Journal Details

This article was published in the following journal.

Name: Biomedical chromatography : BMC
ISSN: 1099-0801
Pages: e4751


DeepDyve research library

PubMed Articles [15944 Associated PubMed Articles listed on BioPortfolio]

Stability indicating RP-HPLC method development and validation for determination of 9 impurities in apixaban tablet dosage forms. Robustness study by Quality by Design approach.

Quality by Design (QbD) based high-resolution HPLC method is described for impurities in Apixaban (APX) in the tablet dosage form. Employing a simple and stability-indicating HPLC method, nine known i...

Synthesis and characterization of oxitropium bromide related substances and novel stability indicating HPLC methods.

The European Pharmacopoeia (Ph. Eur.) described two separate HPLC methods for determination of organic impurities in oxitropium bromide, a synthetic anticholinergic agent used by inhalation in the tre...

HPLC-MS/MS measurement of lidocaine in rat skin and plasma. Application to study the release from medicated plaster.

A simple, sensitive HPLC-MS/MS method was developed and validated for the determination of lidocaine in skin and plasma of rats. The methods were established and validated assessing lower limit of qua...

Fenoldopam mesylate for treating psoriasis: A new indication for an old drug.

Fenoldopam, a highly selective dopamine receptor agonist, is available in clinics as Corlopam™ i.v. for the management of severe hypertension. Recent reports demonstrate its anti-proliferative activ...

Topical antimicrobial peptide formulations for wound healing: Current developments and future prospects.

Antimicrobial peptides (AMPs) are the natural antibiotics recognized for their potent antibacterial and wound healing properties. Bare AMPs have limited activity following topical application attribut...

Clinical Trials [6803 Associated Clinical Trials listed on BioPortfolio]

Lidocaine for Pain Control During Intrauterine Device Insertion

Despite being the most common method of contraception, the use of intrauterine devices (IUDs) is limited by the associated pain during insertion. Many pharmacologic interventions had been ...

A Randomized Trial of Topical Corticosteroid Use in Addition to Oral Antivirals for Prevention of Recurrence of Herpes Simplex Virus (HSV) Keratitis

This is a prospective, randomized clinical trial looking to determine the role of prophylactic treatment with topical corticosteroids in preventing recurrences in patients with a history o...

10% Lidocaine Spray for Intrauterine Device Insertion

Intrauterine device (IUD) insertion is a procedure that can cause pain. Fear of pain during IUD insertion is a barrier to use of this method. Currently, there is no recommended standard me...

Treatment of Acyclovir-Resistant Mucocutaneous Herpes Simplex Disease in Patients With AIDS: Open Label Pilot Study of Topical Trifluridine

To determine the safety, effectiveness, and toxicity of topical (local) trifluridine in treating mucocutaneous (at the nasal, oral, vaginal, and anal openings) Herpes simplex virus ( HSV )...

5% Lidocaine Ointment in the Treatment of Vulvar Vestibulitis

- Study Hypothesis: Use of 5% topical lidocaine ointment will result in improved ability to have sexual intercourse and decreased pain scores in women with vestibulitis when com...

Medical and Biotech [MESH] Definitions

A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions.

A GUANOSINE analog that acts as an antimetabolite. Viruses are especially susceptible. Used especially against herpes.

Fluorides, usually in pastes or gels, used for topical application to reduce the incidence of DENTAL CARIES.

Sequences within RNA that regulate the processing, stability (RNA STABILITY) or translation (TRANSLATION, GENETIC) of RNA.

The application of drug preparations to the surfaces of the body, especially the skin (ADMINISTRATION, CUTANEOUS) or mucous membranes. This method of treatment is used to avoid systemic side effects when high doses are required at a localized area or as an alternative systemic administration route, to avoid hepatic processing for example.

Quick Search

DeepDyve research library

Relevant Topics

An assay is an analytic procedure for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity.  This can be a drug or biochemical substance or a cell in an organism or organic sample. ...

Multiple Sclerosis MS
Multiple sclerosis (MS) is the most common disabling neurological condition affecting 100,000 young adults in the UK. The condition results from autoimmune damage to myelin, causing interference in nerve signaling. Symptoms experienced depend on the pa...

Acne Dermatology Eczema Psoriasis Wound Care Dermatology is the medical specialty concerned with the diagnosis and treatment of skin disorders (Oxford Medical Dictionary). As well as studying how the skin works, dermatology covers...

Searches Linking to this Article