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Basic and editing mechanisms underlying ion transport and regulation in NCX variants.

07:00 EST 22nd November 2019 | BioPortfolio

Summary of "Basic and editing mechanisms underlying ion transport and regulation in NCX variants."

Structure-dynamic analysis of archaeal NCX (NCX_Mj) provided new insights into the underlying mechanisms of ion selectivity, ion-coupled alternating access, ion occlusion, and transport catalysis. This knowledge is relevant, not only for prokaryotic and eukaryotic NCXs, but also for other families belonging to the superfamily of Ca/CA antiporters. In parallel with the ion transport mechanisms, the structure-dynamic determinants of regulatory CBD1 and CBD2 domains have been resolved according to which the Ca-induced allosteric signal is decoded at the two-domain interface and "secondarily" modified by a splicing segment at CBD2. The exon-dependent combinations within the splicing segment control the number of Ca binding sites (from zero to three) at CBD2, as well as the Ca binding affinity and Ca off-rates at both CBDs. The exon-dependent combinations specifically rigidify the local segments at CBDs, yielding the Ca-dependent activation (through Ca binding to CBD1) and Ca-dependent alleviation of Na-induced inactivation (through Ca binding with CBD2). The exon-dependent synergistic interactions between CBDs characteristically differ in NCX1 and NCX3, thereby underscoring the physiological relevance of structure-controlled shaping of ion-dependent regulation in tissue-specific NCX variants. How the ion-dependent regulatory modules operate in conjunction with other regulators (PIP, palmitoylation, XIP, among the others) of NCX is an open question that remains to be determined.

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This article was published in the following journal.

Name: Cell calcium
ISSN: 1532-1991
Pages: 102131

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