Influence of synthesis methods on the internalization of fluorescent gold nanoparticles into glioblastoma stem-like cells.

07:00 EST 23rd November 2019 | BioPortfolio

Summary of "Influence of synthesis methods on the internalization of fluorescent gold nanoparticles into glioblastoma stem-like cells."

Glioblastoma (GBM) is an aggressive disease with currently no satisfying treatment option available. GBM cells with stem cell properties are thought to be responsible for the initiation and propagation of the disease, as well as main contributors to the emergence of therapy resistance. In this work, we developed a novel method to synthesize fluorescent gold nanoparticles as potential drug and gene delivery systems for GBM therapy, able to penetrate three-dimensional stem cell selected patient-derived GBM neurosphere systems in vitro. By using polyethylene imine (PEI) as a stabilizer and reducing agent, as well as fluorescein isothiocyanate (FITC) as a fluorescent marker, our fully in-house developed fluorescent gold nanoparticles (AuPEI-FITC NPs) with core sizes between 3 and 6 nm were obtained via a fast microwave-assisted reaction. Cytotoxicity, adsorption and internalization of AuPEI-FITC NPs into the cell lines JHH520, 407 and GBM1 were investigated using the cellular growth assay and fluorescence-activated cell sorting (FACS) analysis. AuPEI-FITC NPs showed no apparent cytotoxicity and an uptake in cells of up to ~80%. A differentiation between surface-bound and internalized AuPEI-FITC NPs was possible by quenching extracellular signals. This resulted in a maximal internalization degree of 61%, which depends highly on the synthesis method of the nanoparticles and the cell type tested. The best internalization was found for AuPEI-FITC1 which was prepared in a one pot reaction from KAuCl, PEI and FITC. Thus, appropriately synthesized AuPEI-FITC NPs show great potential as vehicles to transport DNA or drugs in GBM cells.


Journal Details

This article was published in the following journal.

Name: Journal of inorganic biochemistry
ISSN: 1873-3344
Pages: 110952


DeepDyve research library

PubMed Articles [20853 Associated PubMed Articles listed on BioPortfolio]

Plant-based gold nanoparticles; a comprehensive review of the decade-long research on synthesis, mechanistic aspects and diverse applications.

The worldwide focus on research in the field of green nanotechnology has resulted in the environmentally and biologically safe applications of a diversity of nanomaterials. Nanotechnology, in general,...

Green Synthesis of Chitosan-Silver/Gold Hybrid Nanoparticles for Biomedical Applications.

Silver and gold nanoparticles (NPs) attract great attention nowadays because of their unique characteristics that entitle them for various biomedical applications. However, there is still a need for s...

Recruitment of various biological macromolecules in fabrication of gold nanoparticles: Overview for preparation and applications.

This review summarizes recent works for fabrication of gold nanoparticles with controlled shape, size, and functional properties using polysaccharides as superior biological macromolecules. Special co...

One-pot synthesis and characterization of ovalbumin-conjugated gold nanoparticles: A comparative study of adjuvanticity against the physical mixture of ovalbumin and gold nanoparticles.

Only few adjuvants are licensed for use in humans and there is a need to develop safe and improved vaccine adjuvants. In this study, we report the one-pot synthesis of antigen ovalbumin (OVA)-conjugat...

DNA Integration with Silver and Gold Nanoparticles. Enhancement of DNA Optical Anisotropy.

DNA integration with silver and gold nanoparticles was carried out by the chemical reduction of silver and gold ions after formation of their complexes with high molecular DNA in solution. It is shown...

Clinical Trials [5566 Associated Clinical Trials listed on BioPortfolio]

Topical Silver Nanoparticles for Microbial Activity

Silver nanoparticles are one of most nanoparticles use nowadays in the research area because it has specific physical and chemical properties, in medical fields silver nanoparticles can in...

Topical Application of Silver Nanoparticles and Oral Pathogens in Ill Patients

The purpose of this study is to determine whether oral topic silver nanoparticles are effective to reduce potential pathogen microbial loads in mechanical ventilation patients.

Topical Fluorescent Nanoparticles Conjugated Somatostatin Analog for Suppression and Bioimaging Breast Cancer

Breast cancer is a communal malignant disease between Saudi females, with a popularity of 21.8%. Since binding to somatostatin receptors (SSTR) induces no immunogenicity in vivo, somatosta...

Fluorescent Cholangiography and Bile Duct Stones

Background. X-ray cholangiography has a high sensitivity and specificity of detecting bile duct stones and is the gold standard. There are no studies describing the sensitivity and specifi...

Fluorescent Thoracoscopy Used in Surgery for Palmar Hyperhidrosis

T3/T4 endoscopic thoracic sympathicotomy is a widely accepted and effective treatment for primary palmar hyperhidrosis. However, sympathetic ganglions are invisible with traditional thorac...

Medical and Biotech [MESH] Definitions

Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.

Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.

Unstable isotopes of gold that decay or disintegrate emitting radiation. Au 185-196, 198-201, and 203 are radioactive gold isotopes.

Stable gold atoms that have the same atomic number as the element gold, but differ in atomic weight. Au-197 is a stable isotope.

A layer of protein coating adsorbed by NANOPARTICLES upon entry into PLASMA or other protein-containing biological fluids, which affects how nanoparticles are internalized by cells and cleared from the body.

Quick Search

DeepDyve research library

Relevant Topics

Stem Cells
Track and monitor developments in stem cell research and commercial development.  Follow the tabs above to read the latest global news, research, clinical trials on stem cells and follow companies active in the stem cell industry.  BioPort...

Drug Discovery
Clinical Approvals Clinical Trials Drug Approvals Drug Delivery Drug Discovery Generics Drugs Prescription Drugs In the fields of medicine, biotechnology and pharmacology, drug discovery is the process by which drugs are dis...

Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Searches Linking to this Article