Topics

The role of cell wall phenolics during the early remodelling of cellulose-deficient maize cells.

07:00 EST 30th November 2019 | BioPortfolio

Summary of "The role of cell wall phenolics during the early remodelling of cellulose-deficient maize cells."

The habituation of cultured cells to cellulose biosynthesis inhibitors such as dichlobenil (dichlorobenzonitrile, DCB) has proven a valuable tool to elucidate the mechanisms involved in plant cell wall structural plasticity. Our group has demonstrated that maize cells cope with DCB through a modified cell wall in which cellulose is replaced by a more extensive network of highly cross-linked feruloylated arabinoxylans. In order to gain further insight into the contribution of phenolics to the early remodelling of cellulose-deficient cell walls, a comparative HPLC-PAD analysis was carried out of hydroxycinnamates esterified into nascent and cell wall polysaccharides obtained from non-habituated (NH) and habituated to low DCB concentrations (1.5 μM; H) maize suspension-cultured cells. Incipient DCB-habituated cell walls showed significantly higher levels of esterified ferulic acid and p-coumaric acid throughout the culture cycle. In terms of cell wall fortification, ferulic acid is associated to arabinoxylan crosslinking whereas the increase of p-coumaric suggests an early lignification response. As expected, the level of hydroxycinnamates esterified into nascent polysaccharides was also higher in DCB-habituated cells indicating an overexpression of phenylpropanoid pathway. Due to their key role in cell wall strengthening, special attention was paid into the dimerization pattern of ferulic acid. A quantitative comparison of diferulate dehydrodimers (DFAs) between cell lines and cell compartments revealed that an extra dimerization took place in H cells when both nascent and mature cell wall polysaccharides were analysed. In addition, qualitative differences in the ferulic acid coupling pattern were detected in H cells, allowing us to suggest that 8-O-4'-DFA and 8-5'-DFA featured the ferulic acid dimerization when it occurred in the protoplasmic and cell wall fractions respectively. Both qualitative and quantitative differences in the phenolic profile between NH and H cells point to a regioselectivity in the ferulate dehydrodimerization.

Affiliation

Journal Details

This article was published in the following journal.

Name: Phytochemistry
ISSN: 1873-3700
Pages: 112219

Links

DeepDyve research library

PubMed Articles [32086 Associated PubMed Articles listed on BioPortfolio]

Directed in vitro evolution of bacterial expansin BsEXLX1 for higher cellulose binding and its consequences for plant cell wall loosening activities.

Expansins are cell wall-loosening proteins found in all land plants and many microbial species. Despite homologous structures, bacterial expansins have much weaker cellulose binding and wall-loosening...

FORCE-ing the shape.

The plant cell wall is a dynamic structure that mediates cell and organ morphogenesis and provides structural support to the whole plant body. The primary load bearing components of the cell wall are ...

Cellulose defects in the Arabidopsis secondary cell wall promote early chloroplast development.

Lincomycin (LIN)-mediated inhibition of protein synthesis in chloroplasts prevents greening of seedlings, represses the activity of photosynthesis-related genes in the nucleus including LHCB1.2, and i...

Leucine-Rich Repeat Extensin Proteins and Their Role in Cell Wall Sensing.

Plant cells are surrounded by a cell wall that provides shape and physically limits cell expansion. To sense the environment and status of cell wall structures, plants have evolved cell wall integrity...

Oryza sativa Brittle Culm 1-like 6 modulates β-glucan levels in the endosperm cell wall.

The endosperm cell wall affects post-harvest grain quality by affecting the mechanical fragility and water absorption of the grain. Therefore, understanding the mechanism underlying endosperm cell wal...

Clinical Trials [14670 Associated Clinical Trials listed on BioPortfolio]

Modulating Effects of Oil Palm Phenolics in Subjects With Pre-diabetes

Study reported that providing Oil Palm Phenolics to Nile rats blocked diabetes progression in this spontaneous diabetes model. The present study aims to determine the beneficial effects of...

Role of Mitochondria in Non Severe Asthma

Asthma is a frequent disease characterized by bronchial hyperresponsiveness, inflammation and remodelling. Bronchial remodelling is an abnormal repair process that contributes to the devel...

Modulating Effects of Oil Palm Phenolics in Uncontrolled Insulin-treated Type 2 Diabetes Mellitus

Study reported that providing Oil Palm Phenolics to Nile rats blocked diabetes progression in this spontaneous diabetes model. The present study aims to determine the beneficial effects o...

Effects of Gallopamil in Severe Asthma

Severe asthma is a difficult to treat disease, characterized by bronchial remodelling, which is an abnormal repair process that contributes to the development of poorly reversible airway n...

Supplementation of Oil Palm Phenolics to Healthy Participants

Our previous study has found that oil palm phenolics (OPP) supplementation at 9 grams per day is safe for consumption. An interesting observation was reported where the consumption of OPP ...

Medical and Biotech [MESH] Definitions

A hexosyltransferase involved in the transfer of disaccharide molecules to the peptidoglycan structure of the CELL WALL SKELETON. It plays an important role in the genesis of the bacterial CELL WALL.

The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.

A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)

A mucoprotein found in the cell wall of various types of bacteria. It has adjuvant and antitumor activities and has been used to augment the production of lymphokine-activated killer (LAK) cells.

Quick Search


DeepDyve research library

Searches Linking to this Article