Using Affinity Purification Coupled with Stable Isotope Labeling by Amino Acids in Cell Culture Quantitative Mass Spectrometry to Identify Novel Interactors/Substrates of Protein Arginine Methyltransferases.

07:00 EST 30th November 2019 | BioPortfolio

Summary of "Using Affinity Purification Coupled with Stable Isotope Labeling by Amino Acids in Cell Culture Quantitative Mass Spectrometry to Identify Novel Interactors/Substrates of Protein Arginine Methyltransferases."

The protein arginine methyltransferase family (PRMT) is known as being the catalytic driving force for arginine methylation. This specific type of post translational modification is extensively used in biological processes, and therefore is highly relevant in the pathology of a profusion of diseases. Since altered PRMT expression or deregulation has been shown to contribute to a vast range of those diseases including cancer, their study is of great interest. Although an increasing number of substrates are being discovered for each PRMT, large scale proteomic methods can be used to identify novel interactors/substrates, further elucidating the role that PRMTs perform in physiological or disease states. Here, we describe the use of affinity purification (AP) coupled with stable isotope labeling with amino acids in cell culture (SILAC) quantitative mass spectrometry (MS) to identify protein interactors and substrates of PRMTs. We also explore the possibility of exploiting the fact most PRMTs display lower dissociation rates with their hypomethylated substrates as a strategy to increase the proportion of substrates identified in AP/MS studies.


Journal Details

This article was published in the following journal.

Name: Methods (San Diego, Calif.)
ISSN: 1095-9130


DeepDyve research library

PubMed Articles [8875 Associated PubMed Articles listed on BioPortfolio]

Effect of different healing stages on stable isotope ratios in skeletal lesions.

Physiological stress is one of the various factors that can have an impact on stable isotope ratios. However, its effect on bone collagen stable isotope ratios is still not fully understood. This stud...

Moiety modeling framework for deriving moiety abundances from mass spectrometry measured isotopologues.

Stable isotope tracing can follow individual atoms through metabolic transformations through the detection of the incorporation of stable isotope within metabolites. This resulting data can be interpr...

Improved strategy for isoleucine H/C methyl labeling in Pichia pastoris.

Site specific methyl labeling combined with methyl TROSY offers a powerful NMR approach to study structure and dynamics of proteins and protein complexes of high molecular weight. Robust and cost-effe...

Synthesis and characterization of natural lanthanum labelled DOTA-Peptides for simulating radioactive Ac-225 labeling.

This research aimed to assess the labeling procedure of Lanthanum to DOTATATE and PSMA-617 peptide for simulating Actinium-225 (Ac) labeling. For this purpose labeling procedure, purification and char...

Selective isotope labeling for NMR structure determination of proteins in complex with unlabeled ligands.

The physiological role of proteins is frequently linked to interactions with non-protein ligands or posttranslational modifications. Structural characterization of these complexes or modified proteins...

Clinical Trials [2390 Associated Clinical Trials listed on BioPortfolio]

Effect of Age, Weight and Sex on Levothyroxine Pharmacokinetics

This study was a prospective, single-center, open-label, non-randomized, pharmacokinetic study using stable isotope carbon-13 labeled levothyroxine

Study of Glutamate and Glutamine Metabolism in Burn Patients Receiving Enteral or Parenteral Nutrition

The purpose of the study is to understand how the body uses amino acids in burned patients during the time they cannot eat normally. Amino acids occur naturally in the body and the food we...

Investigating Optimal Propionate Delivery to the Colon Using Stable Isotope Labeling

Obesity, with its associated co-morbidities, is a major public health challenge. It is estimated that by 2050, 60% of men and 50% of women will be clinically obese. Obesity is associated w...

Exhaled Carbon Monoxide and Red Blood Cell Turnover

Hemoglobin A1C (HbA1c) is the cornerstone of blood sugar monitoring. As HbA1c is formed by the covalent reaction of glucose with hemoglobin throughout the lifespan of the red blood cell (R...

Standard Process 21 Day Purification Program Project

To determine the effects of a commercially available dietary modification plus nutritional supplement regimen, the Standard Process 21 day Purification Program (SPPP), on serum lipids, C-r...

Medical and Biotech [MESH] Definitions

Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.

Hydrogen. The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight 1. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.

Stable gold atoms that have the same atomic number as the element gold, but differ in atomic weight. Au-197 is a stable isotope.

Stable iodine atoms that have the same atomic number as the element iodine, but differ in atomic weight. I-127 is the only naturally occurring stable iodine isotope.

Stable yttrium atoms that have the same atomic number as the element yttrium, but differ in atomic weight. Y-89 is the only naturally occurring stable isotope of yttrium.

Quick Search

DeepDyve research library

Relevant Topics

Cell Culture
Types of Cell Culture Open to view video on cell culture basics. Four catagories of cell culture techniques are listed below: Adherent cells Cells shown to require attachment for growth are sa...

Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Searches Linking to this Article