Vinblastine production by the endophytic fungus Curvularia verruculosa and their in vitro cytotoxicity.

07:00 EST 30th November 2019 | BioPortfolio

Summary of "Vinblastine production by the endophytic fungus Curvularia verruculosa and their in vitro cytotoxicity."

The current study was to isolate endophytic fungi producing high yields of indole alkaloids such as vinblastine analogous to their host Cathranthus roseus. Endophytic fungi were isolated from the leaves of C. roseus, identified as Curvularia verruculosa by molecular techniques, the sequence was deposited in NCBI (MK995628). Vinblastine producing endophytic fungus was grown in 1L Vinca medium for 21 days. The extract was examined for vinblastine by chromatographic techniques. TLC plates showed purple colour spot co migrated with authentic vinblastine and R was calculated by HPTLC (Vin 1 vinblastine -0.75; authentic vinblastine-0.78), these results confirmed vinblastine presence in the Vin1 extract. Further, the TLC purified fungal extract was examined by LC-MS, which revealed the exact mass of vinblastine ([M + H]m/z 811.51). The most important of the study is high yield production of vinblastine; hence, the extract analysed by HPLC revealed 182 μg/L vinblastine. The TLC purified fungal vinblastine was analysed for the cytotoxicity effect on HeLa cell line and it depicted a higher activity with IC-8.5 μg/mL and apoptotic morphological changes were analysed. All the results revealed that the endophytic fungus Curvularia verruculosa produced vinblastine and for the first time in a surplus amount compared to other fungi.


Journal Details

This article was published in the following journal.

Name: Analytical biochemistry
ISSN: 1096-0309
Pages: 113530


DeepDyve research library

PubMed Articles [10761 Associated PubMed Articles listed on BioPortfolio]

Bioactive secondary metabolites from new endophytic fungus Curvularia. sp isolated from Rauwolfia macrophylla.

Over the last decades, endophytic fungi represent a new source of pharmacologically active secondary metabolites based on the underlying assumption that they live symbiotically within their plant host...

Improvement of Curvulamine Production by Precursors Co-addition Strategy in Liquid Culture of Marine-Derived Fungus Curvularia sp. IFB-Z10.

Curvulamine, a novel scaffold alkaloid with remarkable selective antibacterial activity, is produced by marine fungus Curvularia sp. IFB-Z10. However, its deep pharmaceutical research and application ...

Mercury resistance and bioremediation mediated by endophytic fungi.

The present study proposes the use of endophytic fungi for mercury bioremediation in in vitro and host-associated systems. We examined mercury resistance in 32 strains of endophytic fungi grown in cu...

Nonadride and Spirocyclic Anhydride Derivatives from the Plant Endophytic Fungus .

Six new nonadride derivatives (-) and three new spirocyclic anhydride derivatives (-) were isolated from the endophytic fungus obtained from fresh leaves of the toxic medicinal plant The structures ...

Aflatoxins from the endophytic fungus Y-2 isolated from the critically endangered conifer .

Two new [asperxins A () and B ()] and four known (-) aflatoxins were isolated and identified from the endophytic fungus Y-2, which was derived from the needles of the critically endangered conifer . ...

Clinical Trials [2365 Associated Clinical Trials listed on BioPortfolio]

A Phase I Study of Infusional Chemotherapy With the P-Glycoprotein Antagonist PSC 833

The clinical study entitled "A Phase I Study of Infusional Chemotherapy with the P-glycoprotein Antagonist PSC 833" seeks to determine the maximum tolerated dose for a proposed P-glycoprot...

Study of Vinblastine and Sirolimus in Pediatric Patients With Recurrent or Refractory Solid Tumors Including Central Nervous System Tumors

This study is a Phase I study using vinblastine and sirolimus in patients with relapsed solid tumors including selected brain tumors and lymphoma. The investigators hypothesis is that the ...

Vinblastine +/- Bevacizumab in Children With Unresectable or Progressive Low Grade Glioma (LGG)

This is an open-label, randomized, multi-center, comparator Phase II trial looking at the addition of Bevacizumab to Vinblastine in chemotherapy naïve pediatric patients with progressive ...

Pathogenesis and Genetics of Disseminated or Refractory Coccidioidomycosis

Background: - Coccidioidomycosis is caused by a fungus that grows in the southwest United States and parts of Mexico and South America. This disease is caused by breathing dust containing...

Efficacy Safety, and Tolerability of Topical Terbinafine in Patients With Mild to Moderate Toenail Fungus of the Big Toenail

This study is designed to assess the efficacy, safety and tolerability of a topical formulation of terbinafine solution applied daily in patients with toenail fungus. This trial will st...

Medical and Biotech [MESH] Definitions

An imperfect fungus present on most agricultural seeds and often responsible for the spoilage of seeds in bulk storage. It is also used in the production of fermented food or drink, especially in Japan.

A genus of endophytic, ascomycetous mold in the family Trichocomaceae, order EUROTIALES. Anamorphic forms are in the genus PENICILLIUM.

Antitumor alkaloid isolated from Vinca rosea. (Merck, 11th ed.)

An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.

Methods and techniques used to modify or select cells and develop conditions for growing cells for biosynthetic production of molecules (METABOLIC ENGINEERING), for generation of tissue structures and organs in vitro (TISSUE ENGINEERING), or for other BIOENGINEERING research objectives.

Quick Search

DeepDyve research library

Relevant Topic

Multiple Sclerosis MS
Multiple sclerosis (MS) is the most common disabling neurological condition affecting 100,000 young adults in the UK. The condition results from autoimmune damage to myelin, causing interference in nerve signaling. Symptoms experienced depend on the pa...

Searches Linking to this Article