Topics

Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection for Quantitative Analysis of Dried Blood Spots with Unknown Blood Volume.

07:00 EST 3rd December 2019 | BioPortfolio

Summary of "Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection for Quantitative Analysis of Dried Blood Spots with Unknown Blood Volume."

Blood volume in dried blood spot (DBS) analysis is assumed to be constant for DBS punches with fixed area. However, blood volume in the punch is dependent on several factors associated with the blood composition and is preferentially normalized by off-line analysis for quantitative purposes. Instead of using external instrumentation, we present an all-in-one approach for the simultaneous determination of exact blood volume in the DBS punch and the quantitation of target analytes. A DBS is eluted with 500 μL of elution solvent in a sample vial and the eluate is directly subjected to an automated analysis by capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D). The capillary blood volume in the eluate is calculated from the concentrations of the inorganic blood constituents (K+, Na+, or Cl-) determined by CE-C4D, which are linearly proportional to the blood volume originally sampled onto the DBS card. Alternatively, conductivity of the DBS eluate can be used for the blood volume determination by using C4D in a non-separation flow-through mode. The methods are suitable for the determination of blood volume in unknown DBS samples by punching out the entire DBS or by sub-punching a small section of a large DBS with variations of the true vs. the determined volume ≤ 5.5%. Practical suitability was demonstrated by the simultaneous CE-C4D determination of K+ and Na+ (for DBS volume calculation) and amino acids (target analytes) in unknown DBS samples. Quantitative analysis of selected amino acids (related to inborn metabolic disorders) in the unknown DBS was compared with a standard analytical procedure using wet-blood chemistry and an excellent fit was obtained. Use of CE-C4D represents an important milestone in quantitative DBS analysis since the detection technique is universal, the separation technique enables the determination of cations and/or anions and the use of multiple detectors, which further enhance selectivity/sensitivity of the analysis and the range of detectable analytes.

Affiliation

Journal Details

This article was published in the following journal.

Name: Analytical chemistry
ISSN: 1520-6882
Pages:

Links

DeepDyve research library

PubMed Articles [9370 Associated PubMed Articles listed on BioPortfolio]

Double Surfactants-assisted Electromembrane Extraction of Cyromazine and Melamine in Surface Water, Soil and Cucumber samples Followed by Capillary Electrophoresis with Contactless Conductivity Detection.

Cyromazine (CYR) and its main degradation product melamine (MEL) are attracting wide attention due to their potential hazards to the environment and human bodies. In this work, double surfactants-assi...

Analysis of bicarbonate, phosphate and other anions in saliva by capillary electrophoresis with capacitively coupled contactless conductivity detection in diagnostics of gastroesophageal reflux disease.

Bicarbonate and phosphate constitute major salivary buffering components, and their importance consists in the neutralization of acidic gastric contents during reflux episodes. In this work, capillary...

Determination of carbapenem antibiotics using a purpose-made capillary electrophoresis instrument with contactless conductivity detection.

In this study, the employment of a purpose-made capillary electrophoresis (CE) instrument with capacitively coupled contactless conductivity detection (CD) as a simple and cost-effective approach for ...

Thiol-ene Click Derivatization for the Determination of Acrylamide in Potato Products by Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection.

The development of analytical methods for acrylamide formed during food processing is of great significance for food safety, but limited by its inherent characteristics, the analysis of acrylamide is ...

Some thoughts about enantioseparations in capillary electrophoresis.

In this overview the goal of the authors was to analyze from the historical perspective the reasons of success and failure of chiral capillary electrophoresis. In addition, the current trends are anal...

Clinical Trials [2811 Associated Clinical Trials listed on BioPortfolio]

CLUSTER Trial for Outbreak Detection and Response

Despite the critical importance of identifying hospital-associated outbreaks as early as possible in order to limit their spread, there are currently no standardized methods for cluster de...

Capillary Refill for Assessment of Dehydration

Capillary refill is used clinically to assess multiple things in children. This is a prospective observational study of the correlation between capillary refill time and degree of dehydrat...

Evaluation of Capillary Refill Index

Comparison of a peripheral perfusion assessment method using a pulse oximeter to the conventional capillary refill time visually assessed by a clinician.

Electrosclerotherapy for Capillary Malformations

Capillary malformations (port-wine stains) consist of abnormally developed capillary blood vessels in the skin. To date, laser therapy is the only widely accepted treatment modality for ca...

Vestibular Stimulation Therapy for Rhythmic Movement Disorder

This study aims to a) investigate the feasibility of the detection of episodes of RMD using contactless 3D video analysis and customised analysis software and b) the use of vestibular stim...

Medical and Biotech [MESH] Definitions

A separation technique which combines LIQUID CHROMATOGRAPHY and CAPILLARY ELECTROPHORESIS.

A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)

A genotoxicological technique for measuring DNA damage in an individual cell using single-cell gel electrophoresis. Cell DNA fragments assume a "comet with tail" formation on electrophoresis and are detected with an image analysis system. Alkaline assay conditions facilitate sensitive detection of single-strand damage.

Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.

Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.

Quick Search


DeepDyve research library

Searches Linking to this Article