Topics

3D Printing of Large Areas of Highly Ordered Submicron Patterns for Modulating Cell Behavior.

07:00 EST 3rd December 2019 | BioPortfolio

Summary of "3D Printing of Large Areas of Highly Ordered Submicron Patterns for Modulating Cell Behavior."

Fabricating large areas of geometrically complex and precisely controlled topographies is required for the studies of cell behavior on patterned surfaces. Direct laser writing (DLW) is an advanced 3D-fabrication technique, which facilitates the manufacturing of structures within various scales (from a few hundred nanometers to millimeters). However, this method requires improvements in the accuracy and reproducibility of the submicron and nanoscale features that are printed over a large area. Here, we present a scheme to both improve the uniformity of the printed submicron patterns and decrease the printing time. The effects of various processing parameters (e.g., laser power and writing field) on the dimensions and uniformity of submicron pillars as well as on their Young's modulus and surface wettability were assessed. Decreasing the writing field to 33 × 33 µm2 significantly improved the uniformity of submicron pillars that were printed over an area of 4 mm2 in a single-step process. Preosteoblast cells (MC3T3-E1) were used to assess the cytocompatibility of the used material (IP-L780 resin) with a focus on cell morphology, cell proliferation, cytoskeletal organization, and the elastic modulus of the cells. The cells cultured for two days on the submicron pillars showed a polarized shape and a higher Young's modulus of the area corresponding to the nucleus relative to those cultured on flat surfaces. Taken together, the results of the current study clearly show that the submicron patterns created using DLW are both cytocompatible and could modulate the morphology and mechanical properties of cells. This work paves the way for direct printing of submicron features with controlled Young's moduli over large areas in a single-step process, which is necessary for systematically studying how such patterns modulate cellular functions.

Affiliation

Journal Details

This article was published in the following journal.

Name: ACS applied materials & interfaces
ISSN: 1944-8252
Pages:

Links

DeepDyve research library

PubMed Articles [19758 Associated PubMed Articles listed on BioPortfolio]

Siloxane-functionalised surface patterns as templates for the ordered deposition of thin lamellar objects.

A novel method is demonstrated for ordered deposition of thin lamellar objects from a liquid environment onto solid substrates by solid/fluid/solid-driven organisation. Surface functionalisation forms...

3D printing of highly conductive silver architectures enabled to sinter at low temperatures.

Silver (Ag) nanoparticle-based inks are frequently used in printed electronics to form conductive patterns, but often require high-temperature sintering to achieve the optimum electrical conductivity,...

Controllable Liquid-Liquid Printing with Defect-free, Corrosion-Resistance, Unrestricted Wetting Condition.

Conventional printing is worth revisiting because of its established procedures in meeting the surging demand of manufacturing printed electronics, 3D products, etc. However, one goal in penetrating p...

Repeatable and metal-independent nanotransfer printing based on metal oxidation for plasmonic color filters.

Many recently developed nanotransfer printing techniques have received much attention because of their simplicity and low cost. In addition, such techniques are suitable for fabricating nano/microscal...

Screen Printing of a Highly Conductive Graphene Ink for Flexible Printed Electronics.

Conductive inks for the future printed electronics should have the following merits: high conductivity, flexibility, low cost and compatibility with wide range of substrates. However, the state-of-the...

Clinical Trials [6250 Associated Clinical Trials listed on BioPortfolio]

A Study Investigating the Tolerability and Pharmacokinetics of Submicron Budesonide in Children 4 Through 11 Years Old With a History of Mild-To-Moderate Stable Asthma

This Phase 2 study will investigate the tolerability of submicron budesonide at three doses in volunteers with a diagnosis and history of mild-to-moderate stable asthma and evaluate the ph...

Application of 3D Printing Technology in Subtalar Arthrodesis

This study aims to use 3D printing personalized plate to locate the position in which the K-wires would be drilled to save operation time and decrease the number of times that the K-wires ...

Applicability of 3D Printing and 3D Digital Image Reconstruction in the Planning of Complex Liver Surgery (LIV3DPRINT).

Three-dimensional (3D) printing has gained popularity in medicine over the last decade. Once 3D printers have become more affordable, the true strength of this technique has been recognize...

The Effect of Menu Labels on Calories Ordered in Hispanics

Most studies on menu labels have been conducted in mostly non-Hispanic white individuals, and how menu labels affect calories and macronutrients ordered in Hispanics is not clearly underst...

Clinical Application of Personal Designed 3D Printing Implants in Bone Defect Restoration

Objective: Clinical application of 3D printing implant in bone defect treatment. Study design: multicenter randomized controlled study . Methods:patients randomly grouped according to reg...

Medical and Biotech [MESH] Definitions

Arthropods of the order Scorpiones, of which 1500 to 2000 species have been described. The most common live in tropical or subtropical areas. They are nocturnal and feed principally on insects and other arthropods. They are large arachnids but do not attack man spontaneously. They have a venomous sting. Their medical significance varies considerably and is dependent on their habits and venom potency rather than on their size. At most, the sting is equivalent to that of a hornet but certain species possess a highly toxic venom potentially fatal to humans. (From Dorland, 27th ed; Smith, Insects and Other Arthropods of Medical Importance, 1973, p417; Barnes, Invertebrate Zoology, 5th ed, p503)

A genus of large SEA CUCUMBERS possessing the primitive radial configuration of podia in all five ambulacral areas.

A method of analyzing the variation in utilization of health care in small geographic or demographic areas. It often studies, for example, the usage rates for a given service or procedure in several small areas, documenting the variation among the areas. By comparing high- and low-use areas, the analysis attempts to determine whether there is a pattern to such use and to identify variables that are associated with and contribute to the variation.

Patterns of practice in nursing related to provision of services including diagnosis and treatment.

Quick Search


DeepDyve research library

Searches Linking to this Article