Topics

DNA Release from a Modified Electrode Triggered by a Bioelectrocatalytic Process.

07:00 EST 3rd December 2019 | BioPortfolio

Summary of "DNA Release from a Modified Electrode Triggered by a Bioelectrocatalytic Process."

DNA release from an electrode surface was stimulated by application of a mild electrical potential (0 V vs Ag/AgCl). The release process was activated by interfacial pH increase originating from H consumption during O reduction bio-electrocatalyzed by bilirubin oxidase immobilized at the electrode surface. The pH increase resulted in a change of the electrical charge from positive to negative at the surface of SiO nanoparticles (200 nm) associated with the electrode surface and functionalized with trigonelline and boronic acid. While the negatively charged DNA molecules were electrostatically bound to the positively charged surface, the negative charge produced upon O reduction resulted in the DNA repulsion and release from the modified interface. The small electrical potential for O reduction resulting in the interface recharge was allowed due to the bio-electrocatalysis using bilirubin oxidase enzyme. While, in the first set of experiments, the potential was applied on the modified electrode from an electrochemical instrument, later it was generated in situ by biocatalytic or photo-biocatalytic processes at a connected electrode. A multistep biocatalytic cascade generating NADH or photosynthetic process in thylakoid membranes was used to produce in situ a small potential to stimulate the DNA release catalyzed by bilirubin oxidase. The designed system can be used for different release processes triggered by various signals (electrical, biomolecular, and light signals, etc.), thus representing a general interfacial platform for the controlled release of different biomolecules and nanosize species.

Affiliation

Journal Details

This article was published in the following journal.

Name: ACS applied materials & interfaces
ISSN: 1944-8252
Pages:

Links

DeepDyve research library

PubMed Articles [14296 Associated PubMed Articles listed on BioPortfolio]

Power Generation by Selective Self-Assembly of Biocatalysts.

Through a careful chemical and bioelectronic design we have created a system that uses self-assembly of enzyme-nanoparticle hybrids to yield bioelectrocatalytic functionality and to enable the harness...

Sonochemical synthesis and fabrication of honeycomb like zirconium dioxide with chitosan modified electrode for sensitive electrochemical determination of anti-tuberculosis (TB) drug.

Herein, novel honeycomb like zirconium dioxide with chitosan (ZrO@chitosan) nanocomposite have been designed through a facile ultrasound-assisted method and followed by a simple sonication process (ba...

H2O2 production at low over-potentials for electro-enzymatic halogenation reactions.

Various enzymes utilize hydrogen peroxide as oxidant. Such 'peroxizymes' are potentially very attractive catalysts for a broad range of oxidation reactions. Most peroxizymes, however, are inactivated ...

Bioelectrocatalytic performance of d-fructose dehydrogenase.

This review summarizes the bioelectrocatalytic properties of d-fructose dehydrogenase (FDH), while taking into consideration its enzymatic characteristics. FDH is a membrane-bound flavohemo-protein wi...

A boron nitride electrode modified with a nanocomposite prepared from an ionic liquid and tungsten disulfide for voltammetric sensing of 4-aminophenol.

Boron nitride (BN) was used as a support and covered with an ionic liquid (IL) and tungsten disulfide (WS) nanoparticles to obtain an electrode for the determination of 4-aminophenol (4-AP). BN was pr...

Clinical Trials [5585 Associated Clinical Trials listed on BioPortfolio]

A Pharmacokinetic (PK) Study of ACH-0144471 Modified Release Prototype Formulations in Healthy Subjects

The purpose of this study is to evaluate and compare the plasma PK profiles of study drug, ACH-0144471, in healthy subjects after administration of a single dose of multiple ACH-0144471 pr...

A Study Assessing the Safety and Pharmacokinetic Profile of Modified Release Formulations of Tolcapone

The purpose of this study is to assess the pharmacokinetic profiles of tolcapone in healthy subjects after administration of one or two oral doses of multiple modified release prototype fo...

A Study Investigating the Safety, Absorption and Elimination of GLPG3312, a New Drug in the Treatment of Inflammatory Diseases

The purpose of this study is to investigate how safe 2 different formulations (immediate-release (IR) and modified-release (MR) tablets) of the new compound GLPG3312 are and how well they ...

A Conversion Study of Immediate Release (IR) and Modified Release (MR) Forms of Topiramate (TPM)

This multi-center, two-treatment study compares the pharmacokinetic profiles of Immediate Release (IR) and Modified Release (MR) formulations of Topiramate (TPM) in patients with epilepsy.

Bioequivalence Study of Gliclazide 120 mg Modified Release Tablets

The main purpose of this study is to assess comparative bioavailability of two test formulations of Gliclazide 120 mg Modified Release tablets (120 mg gliclazide per modified release table...

Medical and Biotech [MESH] Definitions

An electrochemical technique for measuring the current that flows in solution as a function of an applied voltage. The observed polarographic wave, resulting from the electrochemical response, depends on the way voltage is applied (linear sweep or differential pulse) and the type of electrode used. Usually a mercury drop electrode is used.

Social process whereby the values, attitudes, or institutions of society, such as education, family, religion, and industry become modified. It includes both the natural process and action programs initiated by members of the community.

Food derived from genetically modified organisms (ORGANISMS, GENETICALLY MODIFIED).

A series of progressive, overlapping events, triggered by exposure of the PLATELETS to subendothelial tissue. These events include shape change, adhesiveness, aggregation, and release reactions. When carried through to completion, these events lead to the formation of a stable hemostatic plug.

CELL CYCLE regulatory signaling systems that are triggered by DNA DAMAGE or lack of nutrients during G2 PHASE. When triggered they restrain cells transitioning from G2 phase to M PHASE.

Quick Search


DeepDyve research library

Relevant Topics

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Blood
Blood is a specialized bodily fluid that delivers necessary substances to the body's cells (in animals) – such as nutrients and oxygen – and transports waste products away from those same cells.  In vertebrates, it is composed of blo...

Enzymes
Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...


Searches Linking to this Article