A BMSCs-laden Quercetin/Duck's Feet Collagen/Hydroxyapatite Sponge for Enhanced Bone Regeneration.

07:00 EST 3rd December 2019 | BioPortfolio

Summary of "A BMSCs-laden Quercetin/Duck's Feet Collagen/Hydroxyapatite Sponge for Enhanced Bone Regeneration."

Treating critical-sized bone defects is an important issue in the field of tissue engineering and bone regeneration. From the various biomaterials for bone regeneration, collagen is an important and widely used biomaterial in biomedical applications, hence, it has numerous attractive properties including biocompatibility, hyper elastic behavior, prominent mechanical properties, support cell adhesion, proliferation, and biodegradability. In the present study, collagen was extracted from duck's feet (DC) as a new collagen source and combined with quercetin (Qtn), a type of flavonoids found in apple and onions and has been reported to affect the bone metabolism, for increasing osteogenic differentiation. Further, improving osteoconductive properties of the scaffold Hydroxyapatite (HAp) a biodegradable material was used. We prepared 0, 25, 50 and 100 μM Qtn/DC/HAp sponges using Qtn, DC, and HAp. Their physiochemical characteristics were evaluated using scanning electron microscopy (SEM), compressive strength, porosity, and Fourier transform infrared spectroscopy (FT-IR). To assess the effect of Qtn on osteogenic differentiation, we cultured bone marrow mesenchymal stem cells (BMSCs) on the sponges and evaluated by ALP, MTT assay, and RT-PCR. Additionally, they were studied implanting in rat, analyzed through Micro-CT and histological staining. From our in vitro and in vivo results, we found that Qtn has an effect on bone regeneration. Among the different experimental groups, 25 μM Qtn/DC/HAp sponge was found to be highly increased in cell proliferation and osteogenic differentiation compared with other groups. Therefore, 25 μM Qtn/DC/HAp sponge can be used as an alternative biomaterial for bone regeneration in critical situations. This article is protected by copyright. All rights reserved.


Journal Details

This article was published in the following journal.

Name: Journal of biomedical materials research. Part A
ISSN: 1552-4965


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