Track topics on Twitter Track topics that are important to you
Studying protein-protein interactions (PPIs) is useful for understanding cellular functions and mechanisms. Evaluating these PPIs under conditions as similar as possible to native conditions can be achieved using photo-crosslinking methods because of their on-demand ability to generate reactive species in situ by irradiation with UV light. Various fusion tag, metabolic incorporation, and amber codon suppression approaches using various crosslinkers containing aryl-azide, benzophenone, and diazirines have been applied in live cells. Mass spectroscopy and immunological techniques are used to identify crosslinked proteins based on their capture transient and context-dependent interactions. Here, we discuss various incorporation methods and crosslinkers that have been used for interactome mapping of in live cells.
This article was published in the following journal.
Name: Chembiochem : a European journal of chemical biology
Newly, injectable hydrogels have been renowned as promising biomaterials and appropriate candidates for tissue engineering which can be applied for the development of 3-dimensional cell culture models...
The present work proposes a versatile and efficient method to fabricate rubber nanofiber membranes with controlled morphology and tailored functionality, based on the application of photo-induced thio...
Molecular machines powered by light are appropriately designed multicomponent molecular structures that exploit photochemical processes to perform controlled movements. We discuss the advances in the ...
There is an increasing urge to investigate facile solutions for monitoring biotoxins, which are a major concern in both the food safety and the anti-terrorism fields. Current techniques, such as immun...
Thermoresponsive interpenetrating networks (IPNs) were prepared by sequential synthesis of a biohybrid network of star shaped poly(ethylene glycol) [starPEG] and heparin and a poly(N-isopropylacrylami...
The molecular mechanisms of action of photo(chemo)therapy in skin diseases are investigated in this study. The phototherapeutic modalities employed include UVB (ultraviolet B), UVA (ultrav...
Background: The objective of corneal collagen crosslinking (CXL) is to increase the binding of intrafibrillary and interfibrillary covalent bonds to improve the mechanical stability of the...
This is an amendment for the Research IRB00052103 "Randomized, Split-Body, Single-blinded Clinical Trial of Narrow Band-Ultraviolet B (NB-UVB) Treatment for Vitiligo". Vitiligo is a chron...
The objectives of this study are to evaluate the safety and efficacy of a treatment regimen for high myopia and myopic astigmatism: LASIK followed by crosslinking performed with the KXL Cr...
Antibodies, often monoclonal, in which the two antigen-binding sites are specific for separate ANTIGENIC DETERMINANTS. They are artificial antibodies produced by chemical crosslinking, fusion of HYBRIDOMA cells, or by molecular genetic techniques. They function as the main mediators of targeted cellular cytotoxicity and have been shown to be efficient in the targeting of drugs, toxins, radiolabeled haptens, and effector cells to diseased tissue, primarily tumors.
A non-aqueous co-solvent that serves as tool to study protein folding. It is also used in various pharmaceutical, chemical and engineering applications.
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
A family of crosslinking filament proteins encoded by distinct FLN genes. Filamins are involved in cell adhesion, spreading, and migration, acting as scaffolds for over 90 binding partners including channels, receptors, intracellular signaling molecules and transcription factors. Due to the range of molecular interactions, mutations in FLN genes result in anomalies with moderate to lethal consequences.
Chemical and physical transformation of the biogenic elements from their nucleosynthesis in stars to their incorporation and subsequent modification in planetary bodies and terrestrial biochemistry. It includes the mechanism of incorporation of biogenic elements into complex molecules and molecular systems, leading up to the origin of life.
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...