Photo-crosslinking: An emerging chemical tool for investigating molecular networks in live cells.

07:00 EST 3rd December 2019 | BioPortfolio

Summary of "Photo-crosslinking: An emerging chemical tool for investigating molecular networks in live cells."

Studying protein-protein interactions (PPIs) is useful for understanding cellular functions and mechanisms. Evaluating these PPIs under conditions as similar as possible to native conditions can be achieved using photo-crosslinking methods because of their on-demand ability to generate reactive species in situ by irradiation with UV light. Various fusion tag, metabolic incorporation, and amber codon suppression approaches using various crosslinkers containing aryl-azide, benzophenone, and diazirines have been applied in live cells. Mass spectroscopy and immunological techniques are used to identify crosslinked proteins based on their capture transient and context-dependent interactions. Here, we discuss various incorporation methods and crosslinkers that have been used for interactome mapping of in live cells.


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This article was published in the following journal.

Name: Chembiochem : a European journal of chemical biology
ISSN: 1439-7633


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