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Photo-crosslinking: An emerging chemical tool for investigating molecular networks in live cells.

07:00 EST 3rd December 2019 | BioPortfolio

Summary of "Photo-crosslinking: An emerging chemical tool for investigating molecular networks in live cells."

Studying protein-protein interactions (PPIs) is useful for understanding cellular functions and mechanisms. Evaluating these PPIs under conditions as similar as possible to native conditions can be achieved using photo-crosslinking methods because of their on-demand ability to generate reactive species in situ by irradiation with UV light. Various fusion tag, metabolic incorporation, and amber codon suppression approaches using various crosslinkers containing aryl-azide, benzophenone, and diazirines have been applied in live cells. Mass spectroscopy and immunological techniques are used to identify crosslinked proteins based on their capture transient and context-dependent interactions. Here, we discuss various incorporation methods and crosslinkers that have been used for interactome mapping of in live cells.

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This article was published in the following journal.

Name: Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
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Medical and Biotech [MESH] Definitions

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A non-aqueous co-solvent that serves as tool to study protein folding. It is also used in various pharmaceutical, chemical and engineering applications.

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Chemical and physical transformation of the biogenic elements from their nucleosynthesis in stars to their incorporation and subsequent modification in planetary bodies and terrestrial biochemistry. It includes the mechanism of incorporation of biogenic elements into complex molecules and molecular systems, leading up to the origin of life.

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