Metabolic studies reveal that ruminal microbes of adult steers do not degrade rumen-protected or unprotected L-citrulline.

07:00 EST 12th December 2019 | BioPortfolio

Summary of "Metabolic studies reveal that ruminal microbes of adult steers do not degrade rumen-protected or unprotected L-citrulline."

In vitro and in vivo experiments were conducted to determine the metabolism of rumen-protected or unprotected L-citrulline (Cit) plus L-glutamine (Gln) by ruminal microbes. In the in vitro experiment, whole ruminal fluid (3 mL, containing microorganisms) from steers was incubated at 37oC with 5 mM Cit plus 6 mM Gln (in a rumen-protected or unprotected form) for 0.5, 1, 2, 4 or 6 h after which times 50 µL samples were collected for AA and ammonia analyses. In the in vivo experiment, at 0.5 h before and 0, 0.5, 1, 2, and 4 h after cannulated adult steers consumed 0.56 kg dried-distillers' grain mixed with 70 g Cit plus 70 g Gln (in a rumen-protected or unprotected form) and bermudagrass hay, samples of ruminal fluid and blood were obtained for AA analyses. Results from both invitro and in vivo experiments demonstrated extensive hydrolysis of rumen-unprotected Gln into glutamate, but little degradation of the rumen-protected Gln or rumen-protected and unprotected Cit by ruminal microbes. Concentrations of Cit and arginine in the plasma of steers consuming rumen-protected or unprotected AA increased at 1 and 2 h after the meal, respectively, when compared with values at 0 h. Collectively, these novel findings indicate that ruminal microbes of adult steers do not degrade extra-cellular Cit in a rumen-protected or unprotected form. Our results refute the view that all dietary AA are extensively catabolized by ruminal microorganisms, and also have important implications for dietary supplementation with Cit to ruminants to enhance the concentration of arginine in their plasma and their productivity.


Journal Details

This article was published in the following journal.

Name: Journal of animal science
ISSN: 1525-3163


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