Distribution of CRISPR-Cas Systems in Clinical Carbapenem-Resistant Strains in a Chinese Tertiary Hospital and Its Potential Relationship with Virulence.

07:00 EST 13th December 2019 | BioPortfolio

Summary of "Distribution of CRISPR-Cas Systems in Clinical Carbapenem-Resistant Strains in a Chinese Tertiary Hospital and Its Potential Relationship with Virulence."

In this study, we aimed to characterize the CRISPR-Cas systems in clinical carbapenem-resistant (CRKP) isolates and to investigate the potential association of CRISPR-Cas systems with bacterial virulence. A total of 168 CRKP strains were collected from inpatients in a teaching hospital in Jiangxi Province. Five common carbapenemase genes, subtype genes of the CRISPR-Cas system, and 13 virulence genes were amplified by PCR using specific primers. The potential virulence of all the clinical CRKP strains was tested in a infection model. PCR analysis of five common carbapenemase genes revealed the frequency of carbapenemase gene was the highest in the CRISPR-negative strains, compared to CRISPR type I-E* strains or CRISPR type I-E strains ( < 0.01). Isolates having the subtype I-E* CRISPR-Cas system tended to have more virulence genes such as , , , and , compared to CRISPR-negative isolates and type I-E CRISPR-Cas isolates ( < 0.01). The average survival time of the larvae infected with the isolates having the subtype I-E* CRISPR-Cas system was significantly shorter than the other two group isolates ( < 0.05). The CRKP strains, which had the subtype I-E CRISPR-Cas system or the subtype I-E* CRISPR-Cas system, showed reduced acquisition of carbapenemase genes compared to CRISPR-negative isolates. Importantly, we first found that a small portion of "CR-hvKP" strains were selected from the CRKP clones, which had the type I-E* CRISPR-Cas systems.


Journal Details

This article was published in the following journal.

Name: Microbial drug resistance (Larchmont, N.Y.)
ISSN: 1931-8448


DeepDyve research library

PubMed Articles [34655 Associated PubMed Articles listed on BioPortfolio]

CRISPR-Cas influences the acquisition of antibiotic resistance in Klebsiella pneumoniae.

In the US Carbapenem resistance in Klebsiella pneumoniae (Kp) is primarily attributed to the presence of the genes blaKPC-2 and blaKPC-3, which are transmitted via plasmids. Carbapenem-resistant Kp (C...

Molecular Mechanisms and Epidemiology of Carbapenem-Resistant Isolated from Chinese Patients During 2002-2017.

The emergence and spread of carbapenem-resistant Escherichia coli () pose a serious threat to human health worldwide. This study aimed to investigate the molecular mechanisms underlying carbapenem res...

Emergence of ceftazidime/avibactam resistance in carbapenem-resistant Klebsiella pneumoniae in China.

To investigate the activity of ceftazidime/avibactam (CAZ/AVI) against carbapenem-resistant Klebsiella pneumoniae (CRKP) and identify the resistance mechanisms before CAZ/AVI coming to Chinese market.

Pangenome of uncovers two groups of genomes, one of them with genes involved in CRISPR/Cas defence systems associated with the absence of plasmids and exclusive genes for biofilm formation.

is an opportunistic bacterium that causes hospital-acquired infections with a high mortality and morbidity, since there are strains resistant to virtually any kind of antibiotic. The chase to find nov...

Dissemination of bla harboring Carbapenem-Resistant Acinetobacter baumannii clones in Pakistan.

The rise of carbapenem resistance among Acinetobacter baumannii represents a challenge for the therapeutic management of infections. The present study aimed to investigate the sequence types and carba...

Clinical Trials [8581 Associated Clinical Trials listed on BioPortfolio]

Double Carbapenem as Rescue Strategy for the Treatment of Carbapenemase-Producing Klebsiella Pneumoniae Infections

An observational two-center case-control study exploring the clinical impact of double-carbapenem use in a population of critically il patients with severe carbapenem-resistant Klebsiella ...

Comparison of Bloodstream Infections With Carbapenem Hetero-resistant vs Carbapenem Resistant Klebsiella Pneumoniae

COMBAT trial was contemplated to elucidate unknown clinical relevance of carbapenem heteroresistance among Klebsiella pneumoniae species. Bloodstream infections, type of frequently seen in...

Fecal Microbiota Transplantation for Carbapenem-resistant Enterobacteriaceae

2:1, open-label, single center, randomized controlled trial comparing FMT vs. no intervention for CRE carriers,

Epidemiology of Resistant Microbial Strains Among Different Groups of People (Healthy, Infected and Exposed to Animals)

This study investigates carriage rate and risk factors for acquiring multiresistant bacteria (ESBL producing E.coli and K.pneumoniae, carbapenem-resistant and multidrug resistant P.aerugin...

Impact of Ceftazidime / Avibactam Treatment vs Better Available Therapy on Mortality of Patients With Infections Caused by Carbapenem-resistant Enterobacteria

Patients with infections caused by carbapenem-resistant enterobacteria treated with CAZ-AVI versus patients treated with BAT are compared. The BAT group includes fosfomycin, tigecycline, g...

Medical and Biotech [MESH] Definitions

Strains of Enterobacteriaceae that are resistant to CARBAPENEMS, primarily due to the acquisition of carbapenemase (BETA-LACTAMASE) genes.

Protein components of the CRISPR-CAS SYSTEMS for anti-viral defense in ARCHAEA and BACTERIA. These are proteins that carry out a variety of functions during the creation and expansion of the CRISPR ARRAYS, the capture of new CRISPR SPACERS, biogenesis of SMALL INTERFERING RNA (CRISPR or crRNAs), and the targeting and silencing of invading viruses and plasmids. They include DNA HELICASES; RNA-BINDING PROTEINS; ENDONUCLEASES; and RNA and DNA POLYMERASES.

Adaptive antiviral defense mechanisms, in archaea and bacteria, based on DNA repeat arrays called CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS (CRISPR elements) that function in conjunction with CRISPR-ASSOCIATED PROTEINS (Cas proteins). Several types have been distinguished, including Type I, Type II, and Type III, based on signature motifs of CRISPR-ASSOCIATED PROTEINS.

Strains of the genus Enterococcus that are resistant to the antibiotic VANCOMYCIN. The enterococci become resistant by acquiring plasmids carrying genes for VANCOMYCIN RESISTANCE.

Repetitive nucleic acid sequences that are principal components of the archaeal and bacterial CRISPR-CAS SYSTEMS, which function as adaptive antiviral defense systems.

Quick Search

DeepDyve research library

Relevant Topics

Polymerase Chain Reaction (PCR)
PCR (Polymerase Chain Reaction) uses the ability of DNA polymerase (enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two ident...

Antiretroviral Therapy Clostridium Difficile Ebola HIV & AIDS Infectious Diseases Influenza Malaria Measles Sepsis Swine Flu Tropical Medicine Tuberculosis Infectious diseases are caused by pathogenic...

Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Searches Linking to this Article