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Distribution of CRISPR-Cas Systems in Clinical Carbapenem-Resistant Strains in a Chinese Tertiary Hospital and Its Potential Relationship with Virulence.

07:00 EST 13th December 2019 | BioPortfolio

Summary of "Distribution of CRISPR-Cas Systems in Clinical Carbapenem-Resistant Strains in a Chinese Tertiary Hospital and Its Potential Relationship with Virulence."

In this study, we aimed to characterize the CRISPR-Cas systems in clinical carbapenem-resistant (CRKP) isolates and to investigate the potential association of CRISPR-Cas systems with bacterial virulence. A total of 168 CRKP strains were collected from inpatients in a teaching hospital in Jiangxi Province. Five common carbapenemase genes, subtype genes of the CRISPR-Cas system, and 13 virulence genes were amplified by PCR using specific primers. The potential virulence of all the clinical CRKP strains was tested in a infection model. PCR analysis of five common carbapenemase genes revealed the frequency of carbapenemase gene was the highest in the CRISPR-negative strains, compared to CRISPR type I-E* strains or CRISPR type I-E strains ( < 0.01). Isolates having the subtype I-E* CRISPR-Cas system tended to have more virulence genes such as , , , and , compared to CRISPR-negative isolates and type I-E CRISPR-Cas isolates ( < 0.01). The average survival time of the larvae infected with the isolates having the subtype I-E* CRISPR-Cas system was significantly shorter than the other two group isolates ( < 0.05). The CRKP strains, which had the subtype I-E CRISPR-Cas system or the subtype I-E* CRISPR-Cas system, showed reduced acquisition of carbapenemase genes compared to CRISPR-negative isolates. Importantly, we first found that a small portion of "CR-hvKP" strains were selected from the CRKP clones, which had the type I-E* CRISPR-Cas systems.

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This article was published in the following journal.

Name: Microbial drug resistance (Larchmont, N.Y.)
ISSN: 1931-8448
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