Distinct mA methylome profiles in poly(A) RNA from Xenopus laevis testis and that treated with atrazine.

07:00 EST 10th December 2019 | BioPortfolio

Summary of "Distinct mA methylome profiles in poly(A) RNA from Xenopus laevis testis and that treated with atrazine."

Recent discovery of reversible N-methyladenosine (mA) methylation on messenger RNA (mRNA) and mapping of mA methylomes in mammals, plant and yeast revealed potential regulatory functions of this RNA modification. However, the role of the mA methylomes in amphibious is still poorly understood. Here, we examined the mA transcriptome-wide profile in testis tissues of Xenopus laevis (X. laevis) with and without treatment with 100 μg/L atrazine (AZ) through mA sequencing analysis using the latest Illumina HiSeq sequencer. The results revealed that mA is a highly conserved modification of mRNA in X. laevis. Distinct from that in mammals, mA in X. laevisis enriched around the stop codon and start codon, as is reported in plant. We then investigated the differential expression mA in testes of AZ-exposed X. laevis and compared that with the X. laevis in the control group by mA sequencing. The results indicated that AZ leads to altered expression profile in 1380 mA modification sites (696 upregulated and 684 downregulated). KEGG pathway analysis indicates that the "NOD-like receptors", "tight junction", "Peroxisome proliferator-activated receptors", "adherens junctions", "Glycerophospholipid metabolism" and "Fatty acid biosynthesis" signaling pathways may be associated with abnormal testis development of X. laevis due to exposure to AZ. Analysis results showed a positive correlation between mA modification and mRNA abundance, suggesting a regulatory role of mA in amphibious gene expression. Our first report of mA transcriptome-wide map of an amphibian species X. laevis presented here provides a starting roadmap for uncovering mA functions that may affect/control amphibian testis development.


Journal Details

This article was published in the following journal.

Name: Chemosphere
ISSN: 1879-1298
Pages: 125631


DeepDyve research library

PubMed Articles [4250 Associated PubMed Articles listed on BioPortfolio]

Comparative analysis of the developmental toxicity in Xenopus laevis and Danio rerio induced by Al O nanoparticle exposure.

Engineered aluminum oxide nanoparticles (Al O NPs) having high-grade thermal stability and water dispersion properties are extensively used in different industries and personal care products. Toxicolo...

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles.

The primary visual projection of tadpoles of the aquatic frog Xenopus laevis serves as an excellent model system for studying mechanisms that regulate the development of neuronal connectivity. During ...

Divergent roles of the Wnt/PCP Formin Daam1 in renal ciliogenesis.

Kidneys are composed of numerous ciliated epithelial tubules called nephrons. Each nephron functions to reabsorb nutrients and concentrate waste products into urine. Defects in primary cilia are assoc...

Conservation and divergence of protein pathways in the vertebrate heart.

Heart disease is the leading cause of death in the western world. Attaining a mechanistic understanding of human heart development and homeostasis and the molecular basis of associated disease states ...

2,2',4,4'-tetrabromodipheny ether (BDE-47) disrupts gonadal development of the Africa clawed frog (Xenopus laevis).

Previous studies have shown that BDE-47, one of the most abundant polybrominated diphenyl ethers (PBDEs) congeners, has a weak estrogenic activity, but it has remained unclear whether BDE-47 disrupts ...

Clinical Trials [1372 Associated Clinical Trials listed on BioPortfolio]

PET-MRI for Functional Imaging of the Testis: A Feasibility Study

The primary objective of this study is to explore feasibility of testis functionality assessment and testis imaging obtained from Positron Emissions Tomography (PET) /Magnetic Resonance Im...

Safety Study of FluMist With and Without Ampligen

The purpose of this study is to evaluate FluMist with and without Ampligen in healthy volunteers.

The Incidence of Congenital Undescended Testis Among Dutch Infants

The purpose of this study is to determine the incidence of congenital undescended testis of mature boys, at one year of age, born in the Medical Centre Alkmaar (MCA).

Testis Needle Aspiration of Sperm in Men With Azoospermia

Needle aspiration of the epididymis causes rupture and irreversible damage to the duct. Recurring punctures and needle aspirations of fluid and tissue during Testicular Fine Needle Aspirat...

Retrieval of Sperm From Men With Azoospermia Using Ultrasound-guided Rete Testis Aspiration

The objective of this study is to use ultrasound-guided rete testis flushing and aspiration technique to retrieve sperm, non-surgically, from the testes of azoospermic men. If sperm are re...

Medical and Biotech [MESH] Definitions

Proteins obtained from various species of Xenopus. Included here are proteins from the African clawed frog (XENOPUS LAEVIS). Many of these proteins have been the subject of scientific investigations in the area of MORPHOGENESIS and development.

The commonest and widest ranging species of the clawed "frog" (Xenopus) in Africa. This species is used extensively in research. There is now a significant population in California derived from escaped laboratory animals.

A class of antimicrobial peptides discovered in the skin of XENOPUS LAEVIS. They kill bacteria by permeabilizing cell membranes without exhibiting significant toxicity against mammalian cells.

A family of the order Anura, distinguished by the lack of a tongue. It includes four living genera of aquatic "toads". Two of the most familiar pipids are the popularly called Surinam "toad" (Pipa pipa) and XENOPUS LAEVIS.

A poly(A) binding protein that is involved in promoting the extension of the poly A tails of MRNA. The protein requires a minimum of ten ADENOSINE nucleotides in order for binding to mRNA. Once bound it works in conjunction with CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR to stimulate the rate of poly A synthesis by POLY A POLYMERASE. Once poly-A tails reach around 250 nucleotides in length poly(A) binding protein II no longer stimulates POLYADENYLATION. Mutations within a GCG repeat region in the gene for poly(A) binding protein II have been shown to cause the disease MUSCULAR DYSTROPHY, OCULOPHARYNGEAL.

Quick Search

DeepDyve research library

Relevant Topics

DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. During DNA sequencing, the bases of a small fragment of DNA are sequentially identified from signals emitted as each fragment is re-synthesized from a ...

Gene Expression
The process of gene expression is used by eukaryotes, prokaryotes, and viruses to generate the macromolecular machinery for life. Steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-tran...

Searches Linking to this Article