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Genotyping-by-Sequencing for Analysis of the Genetic Variation of , Incitant of Cucurbit Powdery Mildew.

07:00 EST 30th December 2019 | BioPortfolio

Summary of "Genotyping-by-Sequencing for Analysis of the Genetic Variation of , Incitant of Cucurbit Powdery Mildew."

This research was conducted to identify species causing powdery mildew on cucurbits and to determine genetic variations among isolates of the pathogen. We collected 109 isolates from six cucurbit species hosts (, , , , , and ) in California, Illinois, Indiana, Michigan, New York, Texas, Washington, and Wisconsin in the United States and in Italy. By sequencing the internal transcribed spacer region of the nuclear rDNA of these 109 isolates, was found as the only species causing powdery mildew on cucurbits in the United States. Genotyping-by-sequencing was applied to these 109 isolates to investigate their genetic diversity, which showed a trend of isolates clustering from New York and Italy. In addition, the virulence of 36 isolates was compared and a significant difference ( < 0.0001) was found among them. Furthermore, results of the virulence tests of 28 isolates from Illinois showed significant effects of collection years, hosts, and locations on the virulence of the isolates.

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This article was published in the following journal.

Name: Plant disease
ISSN: 0191-2917
Pages: PDIS03190513RE

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