Excess acetone extraction in silk protein solution greatly accelerates the regeneration progress of silk fibroin for desalting and purification.

07:00 EST 7th January 2020 | BioPortfolio

Summary of "Excess acetone extraction in silk protein solution greatly accelerates the regeneration progress of silk fibroin for desalting and purification."

The dissolution of silk fibroin in highly concentrated neutral salt solution and the subsequent long time-costing desalination have long inhibited silk regeneration. Here, a 4-fold volume of acetone was first added into a silk fibroin of 9.3 M LiBr solution (SF-LBS) to extract the dissolved silk protein. In fact, acetone extracted the salt and water from SF-LBS rather than silk protein. The extracted protein has less than half of the original amount of LiBr and could be redissolved easily in water. The dialysis time of the extracted protein could reduce the original dialysis time by approximately half. SDS-PAGE revealed that acetone extraction didn't induce any breakage to the peptide chains of the extracted silk fibroin. FTIR indicated that the structure of the extracted fibroin was very similar to that of the unextracted fibroin, and displayed predominantly a mixture of random coil and α-helices. X-ray diffraction also showed that acetone extraction had no effect on the crystal structure of the fibroin. In addition, both acetone and LiBr from the processing waste could easily be recycled for silk regeneration. This novel method can significantly enhance the desalination efficiency of highly concentrated LiBr solution containing silk protein without affecting its structure.


Journal Details

This article was published in the following journal.

Name: International journal of biological macromolecules
ISSN: 1879-0003


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Medical and Biotech [MESH] Definitions

A serine-rich sticky protein secreted by MOTHS. Generally, the term refers to silkworm silk gum protein secreted in the middle section of silk gland cells of SILKWORMS, Bombyx mori. Sericin acts as a cement and coating for the two fibroin filaments in a silk strand and is readily soluble in mild alkaline solution.

Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION; CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components.

Hypertonic sodium chloride solution. A solution having an osmotic pressure greater than that of physiologic salt solution (0.9 g NaCl in 100 ml purified water).

The selective extraction of deciduous teeth during the stage of mixed dentition in accordance with the shedding and eruption of the teeth. It is done over an extended period to allow autonomous adjustment to relieve crowding of the dental arches during the eruption of the lateral incisors, canines, and premolars, eventually involving the extraction of the first premolar teeth. (Dorland, 28th ed)

Fibrous proteins secreted by INSECTS and SPIDERS. Generally, the term refers to silkworm fibroin secreted by the silk gland cells of SILKWORMS, Bombyx mori. Spider fibroins are called spidroins or dragline silk fibroins

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