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Flexor tendon injuries and tendinopathy are very common, but remain challenging in clinical treatment. Exosomes-based cell-free therapy appears to be a promising strategy for tendon healing, while limited studies have evaluated its impacts on tenocyte biology. The objective of this study was to characterize a novel purified exosome product (PEP) derived from plasma, as well as to explore its cellular effects on canine tenocyte biology. The transmission electron microscope revealed that exosomes of PEP present cup-shaped structures with the diameters ranged from 80 to 141 nm, and the NanoSight report presented that their size mainly concentrated around 100 nm. The ELISA kits analysis showed that PEP were positive for CD63 and AChE expression, and the cellular uptake of exosomes internalized into tenocyte cytoplasm was observed. The cell growth assays displayed that tenocyte proliferation ability was enhanced by PEP solution in a dose-dependent manner. Tenogenic phenotype was preserved as being evident by that tendon-related genes expression (SCX, COL1A, COL3A1,TNMD, DCN and MKX) were expressed insistently in a high level, while tenocytes were treated with 5% PEP solution. Furthermore, migration capability was maintained and total collagen deposition was increased. More Interesting, dexamethasone-induced cellular apoptosis was attenuated during incubation of tenocytes with 5% PEP solution. These findings will provide the basic understandings about the PEP, and support the potential use of this biological strategy for tendon healing. This article is protected by copyright. All rights reserved.
This article was published in the following journal.
Name: Journal of orthopaedic research : official publication of the Orthopaedic Research Society
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