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High-efficiency Ni-NTA/PAA magnetic beads with specific separation on His-tagged protein.

07:00 EST 1st February 2020 | BioPortfolio

Summary of "High-efficiency Ni-NTA/PAA magnetic beads with specific separation on His-tagged protein."

To effective capture and universal enrichment of His-tagged protein, polyacrylic acid (PAA) brushes were used to encapsulate FeO nanoparticles, connect NTA, and Ni to prepare magnetic beads. These materials provide many advantages, such as excellent stability, tuneable particle size, and a surface for further functionalisation with biomolecules. His-tagged green fluorescence protein (GFP) was separated efficiently, and the binding capacity of FeO/MPS@PAA/NTA-Ni was 93.4 mg/g. Compared with High-Affinity Ni-NTA Resin and Ni-NTA Magnetic Agarose Beads, FeO/MPS@PAA/NTA-Ni nanocomposites exhibited higher separation efficiency and binding capacity towards His-tagged GFP. Moreover, the selectivity and recyclability of them for the target proteins were maintained well after six cycles. This study would widen the application of PAA in constructing multifunctional nanocomposites for biomedical fields.

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This article was published in the following journal.

Name: IET nanobiotechnology
ISSN: 1751-875X
Pages: 67-72

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