Topics

Selective immediate detection of Cu by a pH-sensitive rhodamine-based fluorescence probe in breast cancer cell-line.

07:00 EST 26th December 2019 | BioPortfolio

Summary of "Selective immediate detection of Cu by a pH-sensitive rhodamine-based fluorescence probe in breast cancer cell-line."

A biocompatible fluorogenic chemosensor (N-(3-carboxy acryloyl)) rhodamine B (RHA) comprising a furan-2,5-dione receptor and a rhodamine fluorophore via an iminohydrazine crosslinker has been prepared for more scrutinizing optical properties and utilization in molecular imaging of living cells. The consequences indicated that RHA not only is a good pH indicator in acidic media but also can be used as a great sensitive fluoroionophore with high selectivity. The fluorescence emission of the molecule RHA is quite stable in the presence of 15 diverse cations, 18 anions (especially HPO, S, and F), and 5 amino acids with the different functional group as a model, except Cu ions with observable emission intensity changing. The Cu probe has a dual functional effect, an "on-off" mechanism in solvent media, and an "off-on" mechanism in Phosphate Buffered Saline (PBS) in acidic pH conditions. The detection limits for Cu in acetone-water media was 0.69 μM and 0.18 μM in pH = 5.0. Moreover, RHA can fluorescently and colorimetrically image Cu ions in acidic and neutral pH with low cytotoxicity in living cells.

Affiliation

Journal Details

This article was published in the following journal.

Name: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
ISSN: 1873-3557
Pages: 117989

Links

DeepDyve research library

PubMed Articles [25885 Associated PubMed Articles listed on BioPortfolio]

Indole-based colori/fluorimetric probe for selective detection of Cu and application in living cell imaging.

A highly sensitive and selective indole-based probe IHT exhibited obvious color change from colorless to violet easily detected by naked eye as well as 'turn on' fluorescence response to Cu ion at phy...

A near-infrared xanthene-based fluorescent probe for selective detection of hydrazine and its application in living cells.

Developing fluorescent probes for selective determination of the toxic and carcinogenic hydrazine are pretty significant. Herein, a rhodamine dye coupled to naphthalene was selected as a near-infrared...

Microwave-Assisted One-Pot Synthesis of Indole-Based Fluorescent Probe for Sensitive Detection of Copper (II).

In this study, a rapid, inexpensive and convenient microwave assisted synthesis of indole-3-propionic acid-bisphenol A diglycidyl ether (IPA-SR3) fluorescent probe was developed. This fluorescent prob...

Fluorescent Probes with High pKa Values Based on Traditional, Near-infrared Rhodamine, and Hemicyanine Fluorophores for Sensitive Detection of Lysosomal pH Variations.

Sterically hindered fluorescent probes (A-C) have been developed by introducing 2-aminophenylboronic acid pinacol ester to a traditional, A, a near-infrared rhodamine dye, B, and a near-infrared hemic...

Enhanced sensitivity of fluorescence-based Fe(ii) detection by freezing.

The first example of combining the fluorescent probe-based freeze concentration effect with N-oxide chemistry is reported for the highly sensitive and selective detection of ferrous ion (Fe(ii)). Inte...

Clinical Trials [8384 Associated Clinical Trials listed on BioPortfolio]

The Application of Indocyanine-Green(ICG) Fluorescence Imaging in Hepatocellular Carcinoma

This study aims to evaluate the feasibility of indocyanine green (ICG)-based fluorescence imaging in the detection of liver tumors. By correlating the ICG fluorescence patterns with pathol...

ICG-based Fluorescence Imaging in Localization of Prostate Cancer and Metastatic Lymph Nodes

This study aims to evaluate the feasibility of indocyanine green (ICG)-based fluorescence imaging in the detection of prostate tumors and metastatic lymph nodes. By correlating the ICG flu...

Per-operating Use of a Probe for Detection of β+

The objective of the study is to evaluate the sensibility of the probe Forimtech to detect β+ emission in vivo in humans and its usefulness during elective surgical procedure Hypothesis:T...

Assessment of a New Fluorescence Imaging Technique Using Biopsies From Prostate Resection

In this study, we aim to evaluate the feasibility of anti-PSMA labelling to detect post-operative prostatic tissues by two ex vivo fluorescence techniques. We will evaluate the feasibility...

Radioguided Occult Lesion Localisation by Indocyanine Green

Breast cancer is the most common cancer in women. It is the main cause of cancer deaths among women. The incidence of non-palpable tumors is estimated at 60%. Their better prognosis and th...

Medical and Biotech [MESH] Definitions

Fluoroimmunoassay where detection of the hapten-antibody reaction is based on measurement of the increased polarization of fluorescence-labeled hapten when it is combined with antibody. The assay is very useful for the measurement of small haptenic antigens such as drugs at low concentrations.

A fluorescent probe with low toxicity which is a potent substrate for P-glycoprotein and the bacterial multidrug efflux transporter. It is used to assess mitochondrial bioenergetics in living cells and to measure the efflux activity of P-glycoprotein in both normal and malignant cells. (Leukemia 1997;11(7):1124-30)

Optical imaging techniques used for recording patterns of electrical activity in tissues by monitoring transmembrane potentials via FLUORESCENCE imaging with voltage-sensitive fluorescent dyes.

A molecular probe technique that utilizes branched DNA (bDNA) as a means to amplify the hybridization signal. One end of the bDNA molecule is designed to bind a specific target, while the other end of the bDNA molecule contains many branches of DNA that are designed to bind a probe used for signal detection.

Sensitive method for detection of bacterial endotoxins and endotoxin-like substances that depends on the in vitro gelation of Limulus amebocyte lysate (LAL), prepared from the circulating blood (amebocytes) of the horseshoe crab, by the endotoxin or related compound. Used for detection of endotoxin in body fluids and parenteral pharmaceuticals.

Quick Search


DeepDyve research library

Relevant Topic

Women's Health
Women's Health - key topics include breast cancer, pregnancy, menopause, stroke Follow and track Women's Health News on BioPortfolio: Women's Health News RSS Women'...


Searches Linking to this Article