Selective immediate detection of Cu by a pH-sensitive rhodamine-based fluorescence probe in breast cancer cell-line.

07:00 EST 26th December 2019 | BioPortfolio

Summary of "Selective immediate detection of Cu by a pH-sensitive rhodamine-based fluorescence probe in breast cancer cell-line."

A biocompatible fluorogenic chemosensor (N-(3-carboxy acryloyl)) rhodamine B (RHA) comprising a furan-2,5-dione receptor and a rhodamine fluorophore via an iminohydrazine crosslinker has been prepared for more scrutinizing optical properties and utilization in molecular imaging of living cells. The consequences indicated that RHA not only is a good pH indicator in acidic media but also can be used as a great sensitive fluoroionophore with high selectivity. The fluorescence emission of the molecule RHA is quite stable in the presence of 15 diverse cations, 18 anions (especially HPO, S, and F), and 5 amino acids with the different functional group as a model, except Cu ions with observable emission intensity changing. The Cu probe has a dual functional effect, an "on-off" mechanism in solvent media, and an "off-on" mechanism in Phosphate Buffered Saline (PBS) in acidic pH conditions. The detection limits for Cu in acetone-water media was 0.69 μM and 0.18 μM in pH = 5.0. Moreover, RHA can fluorescently and colorimetrically image Cu ions in acidic and neutral pH with low cytotoxicity in living cells.


Journal Details

This article was published in the following journal.

Name: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
ISSN: 1873-3557
Pages: 117989


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